Bi7072 Bioanalytics II - Analytical methods in clinical praxis

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Jan Havliš, Dr. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
basic knowledge of biochemistry, of analytical, organic and inorganic chemistry are prerequisited.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
main objectives of lecture: introduction into laboratory diagnostics; at the end of the course students should be able - to define relation between bioanalysis and illness diagnosis; - to evaluate and apply respective bioanalytical approaches starting with method selection, through analyte determination to results presentation; - to understand principles of basic methodical approaches and common instrumental techniques.
Syllabus
  • 1. introduction, samples - preanalytical phase
  • 2. instrumentation - analysers; organisation, integration and miniaturisation of analyses
  • 3. quality in clinical analysis - check and control
  • 4. analytical methods - selection and optimisation
  • 5. analytical sets; analytical result - its presentation; selected instrumental methods
  • 6. colourness - physical phenomenon and its utilisation in bioanalysis; medical microbiology
  • 7. clinical immunoanalysis
  • 8. clinical aspects of protein analysis
  • 9. clinical aspects of DNA analysis
  • 10. case study - development of a method for clinical diagnostics; role of buffers in bioanalysis
  • 11. selected analytes 1 - inorganic
  • 12. selected analytes 2 - organic and macromolecules
Literature
  • CHROMÝ, Vratislav. Bioanalytika : analytická chemie v laboratorní medicíně. 1. vyd. Brno: Masarykova univerzita v Brně, 2002, 267 s. ISBN 80-210-2917-X. info
  • CHROMÝ, Vratislav and Jiří FISCHER. Analytické metody v klinické chemii. 1. vyd. Brno: Masarykova univerzita v Brně, 2000, 215 s. ISBN 8021023635. info
Teaching methods
the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because there are no available textbooks in czech language covering certain parts of the subject.
Assessment methods
oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2010, Spring 2011, Spring 2012.