F9601 Optické studium jednotlivých molekul

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/1. 3 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
doc. RNDr. František Vácha, PhD. (assistant)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: doc. Mgr. Karel Kubíček, PhD.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Prerequisites
basic knowledge of fluorescence and phosphorescence, structure of proteins and nucleic acids
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Students of the course will get acquainted with the modern methods for studies of (bio)molecules using advanced spectroscopic and microscopic methods (FRET, UV/Vis, confocal microscopy, etc.)
Syllabus
  • Theoretical background:
    properties of light, interaction of light with matter, emission and absorption processes
  • Physical principles of single-molecule detection:
    spectroscopy and microscopy, light sources, detection and processing of very low signal, basics of low-temperature optical experiments.
  • Single-molecule studies at low temperatures
    molecular physics, solid matrix dynamics, non-linear and quantum optics.
  • Single-molecule studies at room-temperatures
    measurement of life-time of excited states, quantum transitions, polarization spectroscopy, diffusion, chemical reactions, charge transfer, Foerster excited energy transfer
  • Application of single-molecule studies in biophysics, biochemistry and molecular biology:
    enzymatic reaction, protein conformations, diffusion of lipids through membranes, function of ATP-ase, molecular engines, interactions of viruses with cell, photosynthetic systems.
  • Additional single-molecule methods in biological research:
    electron microscopy and single-particle analysis, AFM
Teaching methods
lectures, class discussion
Assessment methods
oral exam
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015.