PřF:Bi2120c Histology practical training - Course Information

Bi2120c Cytology, histology, embryology practical training

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

prof. MUDr. RNDr. Svatopluk Čech, DrSc. (seminar tutor)
prof. MUDr. Drahomír Horký, DrSc. (seminar tutor)
doc. MUDr. Jitka Šťastná, CSc. (seminar tutor)
MUDr. Lenka Krejčířová, Ph.D. (seminar tutor)
MUDr. Irena Lauschová, Ph.D. (seminar tutor)
doc. MUDr. Miroslava Sedláčková, CSc. (seminar tutor)
doc. RNDr. Petr Vaňhara, Ph.D. (seminar tutor)

Guaranteed by

prof. MUDr. Drahomír Horký, DrSc.
Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Contact Person: doc. RNDr. Miroslav Králík, Ph.D.
Supplier department: Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine

Timetable

Tue 7:00–9:50 LF

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Medical Genetics and Molecular Diagnostics (programme PřF, B-BI)

Course objectives

Course objectives
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy.
2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue.
3. Identify tissue types in histologic slides.
4. Describe gamete development, explain principle of meiosis and regulation of gametogenesis.
5. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord.
7. Characterize stages of embryonic development.

Syllabus

• Introduction, organization of practicals, teaching aids. Overview of histological methods. Tissue processing for light microscopy - explanation and film. Basic histochemistry and immunocytochemistry.
• Cytology: Ultrastructure of the cell nucleus (during interphase and mitosis). Ultrastructure of cell organelles (mitochondria, the Golgi apparatus, the endoplasmic reticulum, ribosomes, lysosomes, peroxisomes, and the centriol). Cell inclusions. The arrangement of cell surfaces: apical, lateral, and basal ones. Intercellular junctions: adhering, occluding, and communicating ones.
• Blood elements: Preparation of blood smears and their staining. Cytology of formed elements of blood. Differential white cell count (dWCC) - average values, abnormities and terminology of dWCC. Slide: A smear of peripheral blood stained by Pappenheim's method.
• Hematopoiesis: Erythropoiesis, granulopoiesis and development of thrombocytes. Development of lymphocytes and monocytes. Microscopic structure of the bone marrow. Slide: A smear of bone marrow stained by Pappenheim's method.
• Embryology I. Interactive repetition of early human development. Outline of the development of fetal membranes: amnion and chorion. Placenta, types of placenta, development of placenta in humans. Structure and function of human placenta. Development of umbilical cord. Anomalies of placenta and umbilical cord. Uterus and pregnancy. Development of external form of the embryo.
• Embryology II. Interactive repetition of Embryology I. Parturition. FMarks of full-term and mature fetus. The rule of Haase. Position, posture, habitus and presentation of the fetus. Multiple pregnancy: mono- and dizygotic twins, arrangements of fetal membranes. Prenatal diagnostic and medicine. Essentials of teratology. Critical periods.
• General histology: Use of the light microscope, common faults in microscopy. Results of basic staining methods in histology: Hematoxylin-eosine /HE/ (hepar), hematoxylin-eosine-safron /HES/ (oesophagus), AZAN (prostate), impregnation (cerebellum).
• Connective tissue proper - classification and examples. Slides: Funiculus umbilicalis, Oesophagus, Posterior segment of the eye, Lien, Aorta.
• Supporting tissues: cartilage and bone. Histogenesis of the bone tissue (ossification). Slides: Trachea, aAricula, Elastic cartilage, Lamellar bone, Chondrogenic ossification.
• Epithelial tissue - epithelial membranes, glandular epithelium. (covering epithelia). Slides: Ren, Vesica fellea, Trachea, Oesophagus, Urether, Palpebra, Intestinum tenue, Glandula parotis, Glandula submandibularis.
• Muscle tissue. Slides: Apex linguae, Intestinum crassum, Myocardium.
• Nerve tissue. Slides: Cortex cerebri, Cerebellum, Medulla spinalis, Ganglion spinale, Peripheral nerve, Motor end plate.

Literature

• ČECH, Svatopluk, Drahomír HORKÝ, Irena LAUSCHOVÁ, Miroslava SEDLÁČKOVÁ and Jitka ŠŤASTNÁ. Histologická praktika a metody vyšetřování tkání a orgánů (Histological practicals and methods of investigation of tissues and organs). 1.dotisk 1. vydání. Brno: Vydavatelství MU Brno-Kraví hora, 2002, 162 pp. ISBN 80-210-1774-0. info
• ČECH, Svatopluk, Drahomír HORKÝ, Ladislav ILKOVICS, Irena LAUSCHOVÁ, Miroslava SEDLÁČKOVÁ and Jitka ŠŤASTNÁ. Histologická praktika a laboratorní technika. Učební text a atlas (Histological practicals and technique. Textbook and Atlas). 1. vydání. Brno: Multimediální text podpořený FRVŠ č. projektu 357/2006 (F3/d), 2006. URL info

Teaching methods

Content of this course includes study of slides and electron micrographs in microscopic hall as well as class discussion on the base of materials in the text book.

Assessment methods

A precondition for obtaining the course-unit credit is full attendance and demonstration of the basic knowledge of the semester schoolwork in the form of 3 brief oral exams, each is evaluated by 100, 60, 25 or 0 points. A minimum 180 points is required for fulfillment.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

• Enrolment Statistics (Spring 2012, recent)
  
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