C8980 Protein Preparation and Characterization I - Expression and Purification

Faculty of Science
Spring 2013
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
RNDr. Lubomír Janda, Ph.D. (lecturer)
Ing. Blanka Pekárová, Ph.D. (lecturer)
Mgr. Radka Dopitová, Ph.D. (lecturer)
prof. Mgr. Lukáš Žídek, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: RNDr. Lubomír Janda, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 8:00–9:50 C05/114
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 45 fields of study the course is directly associated with, display
Course objectives
This course is for students to gain the basic knowledge about the preparation of proteins in sufficient quantity and quality for crystallization, NMR analysis and others biochemical measurements. At the end of this course the student will be able to: understand the different approaches in the cloning of various expression vectors using affinity tags or without tags, and subsequent purification of the recombinant protein with affinity chromatography or classically; to remove the fusion protein (anchor); to hold the protein in soluble state; to know various methods for protein detection during the purification procedures.
Syllabus
  • 1. The molecular principles for understanding proteins. 2. Calculation in the molecular biosciences 3. The important properties of proteins and how to explore them. 4. DNA cloning. 5. Protein expression. 6. Cell disruption. 7. Fusion proteins and affinity purification. 8. Protein purification chromatography. 9. Protein quality. 10 Analytical methods used to study proteins. 11. Protein labelling. 12. Invited lecture.
Teaching methods
The main teaching method are lectures that include particular examples from the own scientific praxis of participating techers and demonstration of the solution of particular problems.
Assessment methods
written test + oral exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2012, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2013, recent)
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