FA603 Electron microscopy in biology

Faculty of Science
Spring 2013
Extent and Intensity
1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
Teacher(s)
Ing. Jana Nebesářová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: prof. Dr. Jiří Kozelka, PhD.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • Applied Physics (programme PřF, B-AF, specialization Medical Physics)
  • Biophysics (programme PřF, B-FY) (2)
  • Biophysics (programme PřF, N-FY, specialization Aplikovaná biofyzika) (2)
  • Biophysics (programme PřF, N-FY, specialization Molekulární biofyzika) (2)
Course objectives
The course introduces basic physical principles of transmission and scanning electron microscopy, and preparation methods of biological objects for electron microscopy.
Syllabus
  • Physical principles of electron microscopy and physical properties of accelerated electrons, resolution, magnification
  • Transmission electron microscope, electromagnetic lenses and defects, construction of the microscope
  • Preparation of specimens for TEM, physical methods, cryomethods, microwaves
  • Preparation of specimens for TEM, chemical methods, fixation, dehydration, embedding
  • Preparation of ultra-light cuts, ultramicrotome, knives, contrasting, underlying films
  • Scanning electron microscopy, construction of the microscope
  • Preparation of specimens for SEM, drying, coating
  • Electron microscopy cytology
  • Artifacts caused by TEM and SEM preparation, by microscope, photographic process
  • Microscopic images, digital and classical photography
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018.
  • Enrolment Statistics (Spring 2013, recent)
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