PřF:Bi8870 Mechanisms of cell death - Course Information

Bi8870 Mechanisms of cell death, function, methods

Extent and Intensity

2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Hyršlová Vaculová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

there are 12 fields of study the course is directly associated with, display

Course objectives

Students of the course will acquire knowledge and detailed survey about the role and molecular mechanisms of regulation of cell death, especially apoptosis, in multicellular organisms. Students will understand basic signaling pathways responsible for initiation/execution of apoptosis, and obtain information about individual crucial molecules and their function in modulation of cell death. They will be familiarized with the important role of cell death/apoptosis in development and treatment of various diseases, especially cancer. Finally, students will be provided with a detailed characteristics of modern methods currently available for detection of cell death, their practical applications, and general recommendations.

Syllabus

• 1) Introduction to cell death field, definition, classification of individual cell death forms, main biochemical and morphological features, physiological importance of cell death in the development and homeostasis of multicellular organisms. Model organisms in apoptosis study, main signaling pathways and apoptosis inductors. 2) Proteases in cell death regulation (caspases, non-caspase proteases, metacaspases). Caspases – definition, characteristics, structure, function, classification, role of individual caspases in apoptosis regulation, caspases substrates and inhibitors. Non-caspase proteases – cathepsins, calpains, granzymes, and their functions. Metacaspases, their structure and function. 3) Mitochondria and nucleus in regulation of apoptosis. Mitochondria: function in apoptosis, oxidative metabolism, mitochondrial membrane permeabilisation and potential, pro- and anti-apoptotic Bcl-2 family proteins, their structure and function. Proapoptotic proteins released from mitochondria. Nucleus: apoptotic biochemical and structural changes of nucleus. Organelle cross-talk during apoptosis. 4) Intrinsic apoptotic pathway. DNA damaging drugs. The role of specific kinases in DNA damage response. The role of p53 protein. Apoptosis and cell cycle. 5) Extrinsic apoptotic pathway. Description of death receptor-mediated signaling pathway, characterisation of death receptors and ligands of TNF family. Physiological role of these ligands and their importance in therapy. Important molecules in extrinsic pathway. TRAIL – a model inductor of extrinsic apoptotic pathway and promising candidate for anticancer therapy. TRAIL-induced signaling pathway. Physiological importance of TRAIL. Molecular mechanisms responsible for regulation of cell sensitivity/resistance to TRAIL. TRAIL in anticancer therapy, new applications. 6) Interaction of extrinsic and intrinsic apoptotic pathways, importance, impact, individual levels. Combined therapy in cancer, principles and applications. New approaches and current research in the field. 7) Apoptosis in disease. Examples of individual diseases related to deregulated apoptosis, and the consequences. New therapeutic possibilities of apoptosis regulation. 8)Apoptosis and cancer – news and future prospects. 9) Characterisation and comparison of individual cell death forms, definitions, description, classification, regulation, significance. Necrosis, autophagy, anoikis, etc. 10) Methods for apoptosis detection – modern methods of cell and molecular biology and biochemistry. Apoptosis detection at the level of cell populations, individual cells, and cellular organelles and molecules. In vitro and in vivo detections. Detailed survey of currently available methods. 11) Principles of selected apoptosis detection methods, advantages, limitations, criteria for selection, applications. Data interpretation. Methods for detection of other cell death forms, principles, individual examples. Discussion 12) Future and new trends in cell death/apoptosis field. Hot topics in apoptosis field. Discussion.

Literature

• bude specifikováno v prubehu vyuky
• další odkazy na literaturu poskytovány přímo během přednášek
• Cell death. Edited by Gerry Melino - David Vaux. 1st pub. Hoboken, NJ: Wiley-Blackwell, 2010, xi, 303. ISBN 9780470715734. info

Teaching methods

lectures accompanied by discussions

Assessment methods

The course will be completed by the written exam, combination of the test questions and the written answers, based on the content of the lectures from the whole student term. In order to pass the exam, the student has to obtain 40 points out of the 70.

Language of instruction

Czech

Follow-Up Courses

• Bi8870c Mechanisms of cell death - practical lesson

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!

Listed among pre-requisites of other courses

• Bi8870en Mechanisms of Cell Death
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• Enrolment Statistics (Spring 2014, recent)
  
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