C7075 Bioanalytical chemistry in laboratory medicine

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
Mgr. Miroslava Bittová, Ph.D. (lecturer)
prof. Ing. Vratislav Chromý, CSc. (lecturer)
Guaranteed by
prof. Ing. Vratislav Chromý, CSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Prerequisites
Basic knowledges of inorganic, organic and analytical chemistry, biochemistry and fhysical chemistry are required. Lescture enlarges analytical chemistry to the field of biological samples based namely on enzymatic immunoanalytic methods with the used of instrumental techniques. Studens-absolvents in the 3rd studying year are preferred.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Lescture enlarges analytical chemistry to the field of biological samples based namely on enzymatic immunoanalytic methods with the used of instrumental techniques. Studens-absolvents in the 3rd studying year are preferred.
Syllabus
  • 1. The aim of clinical chemistry and the position of a clinical analyst. Specificity of biological samples and the influence of biological matrix. The main methods of withdrawal of biological specimens. How to express and interpret an analytical result. Acute/POCT analyses. 2. The selection of an analytical method (general, specific, analytical and clinical features). Primary, reference, recommended and routine methods. Calibration, control and reference materials. Work organization, instruments, analyzers. Preanalytical phase: withdrawal, transport, storage and preparation of laboratory specimens. 3. Quality management in clinical laboratory (intra- and interlaboratory qualiy control, tolerance limits). The importance of a positive evaluation of clinical laboratory. Buffers for clinical chemistry (basic features, compatibility). 4. Basic/vital aminoacids, peptides and proteins and their significance. Methods of their determination. 5. Enzyme analyses: kinetics, temperature, accelerators/retarders, buffers and substrates. Enzyme as a bioanalyte: the measurement of its catalytic concentration/enzymatic substrates. Enzymes as analytical reagents/accelerators for other bioanalytes. 6. Immunochemistry and immunoanalysis: serologic, precipitation and immunodiffusion. Methods with labeled reactants: radionuclides, enzymes, fluorophores, luminophores etc. Competitive/uncompetitive methods, homogeneous/heterogeneous methods. The use of avidin and biotin. 7. Analytical importance of nucleic acids (structure/properties), important NA-sequences. Polymerase chain reaction and its use for diagnostics of patogens in forensic/prenatal diagnostics. 8. Colour properties of molecules. Basic rules: conjugated double-bonds, aromatic/ heterocyclic compounds, substituents and their effect on polarization/ionization of the molecule, planar structure, oxidation/reduction reactions. Examples for the use in clinical chemistry. 9. Basic indicating reactions: coupling, oxidation coupling with peroxide, oxidation of leuco bases. The reactions with coenzymes of NAD/NADP, tetrazolium salts, formazans. 10. Development/production of analytical kits for in vitro diagnostics (requirements, production procedures). 11. Development/perspectives of clinical chemistry: consolidated laboratories, diagnostic centres, method miniaturisation, microchips, bio-probes, laboratory automation. 12. Some selected bioanalytes, their significance/determination: glucose, urea, uric acid, creatinine, bilirubin, cholesterol, triacylglycerols, total protein/albumin, fibrinogen, hemoglobin, chlorides, iron, enzymes (amylase, aminotransferases, acid/alkaline phosphatase, chlinesterase, creatinkinase, GGT), insulin, lipids, prostate specific antigen, gravidity test. The use of PCR for the estimation of TBC And boreliosis. 13. Visit of a clinical laboratory, principles of quality control/management.
Literature
  • CHROMÝ, Vratislav, Luděk DOHNAL, Alena FISCHEROVÁ, Eva KLOKOČNÍKOVÁ, Josef KRATOCHVÍLA, Zdenek KUBÍČEK, Miloš POLLAK, Milena RIKANOVÁ, Petr SCHNEIDERKA, Luděk ŠPRONGL, Dana TRÁVNÍČKOVÁ and Jan VILÍMEC. Management kvality v analytické a klinické chemii (Quality management in analytical and clinical chemistry). první. Brno: Masarykova univerzity, Brno, PřF, Ústav chemie, 2009, 215 pp. ISBN 978-80-903732-6-6. info
  • CHROMÝ, Vratislav. Bioanalytika : analytická chemie v laboratorní medicíně. 1. vyd. Brno: Masarykova univerzita v Brně, 2002, 267 s. ISBN 80-210-2917-X. info
  • CHROMÝ, Vratislav and Jiří FISCHER. Analytické metody v klinické chemii (Analytical methods in clinical chemistry). Brno: MU Brno, 2000, 211 pp. ISBN 80-210-2363-5. info
Teaching methods
oral lecture
Assessment methods
test and oral exam
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught: every week.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2014, recent)
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