C8024 Separation Methods B

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Jan Havliš, Dr. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
C7023 Separation Methods A
basic knowledge of physical and analytical chemistry + subject C7023 separation methods A are pre-requisited.
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
main objectives of lecture: introduction into macromolecular and electromigration analytical separation methods; mastering their principles; acquainting with the wide scope of their contemporary implementation and utilisation; choice and optimisation of separation system, evaluation of their efficiency and evaluation of the separation results.
Syllabus
  • separation of macromolecules: history, theoretical base – description of macromolecule, molecular mass; modes of macromolecule separation – GPC (gel permeation chromatography), carry-out of GPC – columns, detectors (light scattering), HC (hydrodynamic chromatography), FFF (force field fractionation) – principles, instrumentation, modes (SdFFF, ThFFF, EFFF, FFFF)
  • gas chromatography (GC) – history, theoretical base (isotherms, gas compressibility, retention), GC arrangement, mobile phase delivery; injection (splitless, with split); separation column, stationary phase types (WCOT, FSOT, SCOT, PLOT), detectors (flame ionisation, chemiluminiscence, mass spectrometric, etc.).
  • electromigration methods, history and theory, ion mobility, electroosmotic flow, separation description, efficiency and influencing factors (heat, diffusion, dispersion); instrumentation – injection, voltage sources, separation channel (capillary, membrane, gel, chip), detectors (photometric, laser induced fluorescence, conductivity detection, mass spectrometry etc.); preparative modes; electrophoretic system definition and analytic information in electrophoretogram; basic modes of electromigration methods – capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), slab gel electrophoresis (GE), capillary isoelectric focusation (CIEF), capillary isotachophoresis (CITF), micellar electrokinetic chromatography, thin-layer elektrochromatography, capillary electrochromatography; affinity and non-aqueous electrophoreses
Literature
  • Capillary electrophoresis : methods and protocols. Edited by Philippe Schmitt-Kopplin. Totowa, N.J.: Humana Press, 2008, xix, 809. ISBN 9781588295392. info
  • Analysis and detection by capillary electrophoresis. Edited by M. L. Marina - M. Valcárcel Cases - Ángel Ríos Castro. 1st ed. Amsterdam: Elsevier, 2005, xxviii, 76. ISBN 0444517189. info
  • PLEVA, Michael A. and Frank A. SETTLE. Gas chromatography - mass spectrometry a knowledge base. Amsterdam: Elsevier, 1988, 12 s. ISBN 0444427619. info
  • AMBROSE, D. Gas chromatography. 2nd ed. London: Butterworths, 1971, 321 s. ISBN 0-408-70129-3. info
Teaching methods
the lecture is based on ppt presentation and its explication. presentation itself will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in English covering certain parts of the subject.
Assessment methods
oral examination; students are required to understand and to be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
Language of instruction
English
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2014, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2014/C8024