Bi4010c Essential molecular biology - practice

Faculty of Science
Spring 2015
Extent and Intensity
0/1. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 11:00–11:50 B11/132
Prerequisites
! Bi4020c Essential methods in mol. biol && NOW( Bi4010 Essential molecular biology )
Basic knowledge of general genetics, physiology, anorganic chemistry, organic chemistry and biochemistry.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 10 fields of study the course is directly associated with, display
Course objectives
This lecture is conceived as an introduction into molecular biology methods and it is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who are not specialized in molecular biology. Understanding of principle and practical use of molecular biology methods is emphasized. At the end of the course, students should be able to understand principles of the methods and make reasoned decisions about choosing the right methodological approaches to address specific biological phenomena.
Syllabus
  • 1. Structure of nucleic acids - seminar (primary, secondary and tertiary structure of DNA and RNA, different conformations of DNA and their significance in biological systems).
  • 2. Nucleic acids: manipulation, centrifugation, modifications in vitro.
  • 3. Electrophoretic analyses of nucleic acids.
  • 4. Hybridisation of nucleic acids: probe preparation, labelling.
  • 5. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
  • 6. Sequence analyses of nucleic acids, genome sequencing.
  • 7. DNA cloning (characterization and using of basic types of vectors, cloning strategies, DNA transfer to prokaryotic cells).
  • 8. Polymerase chain reaction.
  • 9. Basic methods of molecular diagnostics.
  • 10. Basic methods of bioinformatics.
Literature
  • BROWN, T. A. Gene cloning and DNA analysis : an introduction. 5th ed. Oxford: Blackwell Publishing, 2006, xx, 386. ISBN 1405111216. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • ROSYPAL, Stanislav. Úvod do molekulární biologie. Třetí inovované vydání. Brno, 1998, 300 pp. Díl I. Informační makromolekuly. ISBN 80-902562-0-1. URL info
Teaching methods
Weekly lectures using powerpoint presentations and demonstration of selected techniques.
Assessment methods
Examination consists written test containing 30 questions.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Studenti profilující své studium směrem k využití molekulárně biologických metod si mohou namísto předmětu Bi4010 zapsat jednu z dvojic předmětů Bi4020 + Bi4020c nebo Bi4020 + Bi4035, která jim bude uznána jakožto předmětový ekvivalent Bi4010.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2015, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2015/Bi4010c