S1007 Doing structural biology with the electron microscope

Faculty of Science
Spring 2016
Extent and Intensity
2/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Mgr. Jiří Nováček, Ph.D. (lecturer)
Dr. rer. nat. Jürgen Plitzko (lecturer)
Dr. Tanvir Shaikh (lecturer)
Guaranteed by
Dr. rer. nat. Jürgen Plitzko
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable
Mon 14:30–16:30 E35/211
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Introduction to Structural Biology with the Electron Microscope
Fundamentals of Electron Microscopy - electron optics/electron diffraction
Contrast and Image Formation in the Electron Microscope
Sample Preparation for TEM
3D Reconstruction and Image Processing
Electron Tomography
Single-Particle Analysis
Syllabus
  • The course will cover preparation methods for electron microscopy with a focus on preserving native structures (purified and isolated molecules cells and tissue) within the high vacuum of the electron microscope. Hardware and software requirements to do imaging with the electron microscope for structural and molecular biology and computational tools and image processing methods to extract three-dimensional (3DEM) information from electron micrographs. Additional topics include correlative methods to image biological structures on different length scales, e.g. light and fluorescence microscopy and X-ray imaging and tomography.
Literature
    recommended literature
  • Grant Jensen Methods in Enzymology, Volume 481: Cryo-EM, Part A: Sample Preparation and Data Collection
  • Grant Jensen Methods in Enzymology, Volume 481: Cryo-EM, Part B: 3-D Reconstruction'
  • Joachim Frank, 'Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell'
  • Three-dimensional electron microscopy of macromolecular assembliesvisualization of biological molecules in their native state. Edited by J. Frank. 2nd ed. New York: Oxford University Press, 2006, xiv, 410 p. ISBN 0195182189. info
Teaching methods
lectures
Assessment methods
exam
Language of instruction
English
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms spring 2012 - acreditation, Spring 2015, Spring 2017, spring 2018.
  • Enrolment Statistics (Spring 2016, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2016/S1007