PřF:Bi4035 Molecular biology - lab.pract. - Course Information
Bi4035 Molecular biology - laboratory practice
Faculty of ScienceSpring 2017
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
doc. Mgr. Petr Beneš, Ph.D. (seminar tutor)
Mgr. Jarmila Navrátilová, Ph.D. (seminar tutor)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
Mgr. Blanka Jančeková, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi4035/01_1: Mon 20. 2. to Mon 22. 5. Wed 13:00–16:50 D36/216, V. Růžičková
Bi4035/01_2: Mon 20. 2. to Mon 22. 5. Wed 13:00–16:50 D36/216, V. Růžičková
Bi4035/02_1: Mon 20. 2. to Mon 22. 5. Thu 13:00–16:50 D36/216, T. Botka
Bi4035/02_2: Mon 20. 2. to Mon 22. 5. Thu 13:00–16:50 D36/216, P. Beneš
Bi4035/03_1: Mon 20. 2. to Mon 22. 5. Wed 9:00–12:50 D36/216, P. Beneš
Bi4035/03_2: Mon 20. 2. to Mon 22. 5. Wed 9:00–12:50 D36/216, J. Navrátilová - Prerequisites
- ( C1600 Laboratory in Chemistry || C1080 General Chemistry-lab.course || C1620 General and Inorg.Chem.-lab. || C1650 Fundamentals of Chem.-lab. || C2100 Inorganic Chemistry-lab.course || C2500 General Chemistry-lab.course || C2740 Fundament. of Chem.-lab.course || C2480 Basic Organic Chem. and Bioch. || Bi9410 Structural Biology || SOUHLAS) && ( Bi4020 Molecular biology || NOW( Bi4020 Molecular biology )) && ! Bi4020c Essential methods in mol. biol
Znalost molekulární struktury a funkce prokaryotického a eukaryotického genomu. Znalost základních mikrobiologických technik jako kultivace bakterií, stanovení titru bakteriální suspenze. Znalost výpočtů složení chemických roztoků a jejich příprava. Předpokládá se předchozí absolvování základních cvičení z anorganické a organické chemie a mikrobiologie. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 150 student(s).
Current registration and enrolment status: enrolled: 0/150, only registered: 0/150 - fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course students should be able to competently analyse the genetic material including chromosome DNA and solve experiments with plasmids originating from the bacteria.
- Learning outcomes
- Student will be able to:
isolate plasmid DNA of sufficient quality and quantity;
analyze isolated genetic material by basic methods of molecular biology such as restriction endonuclease cleavage and ELFO;
prepare sufficient amount of PCR amplicon of the gene of interest;
create the construct of the plasmid vector and the insert of the gene of interest and perform the transformation of competent cells;
use theoretical knowledge of other methods and principles in subsequent laboratory practice - Syllabus
- This course include the practical tasks which serve to acquire the basic methods in the molecular biology, primarily, isolation and manipulation with nucleic acids. Preparation, solutions useful for molecular methods: Isolation of plasmid DNA Concentration and purity of DNA Restriction splitting, ELFO Ultracentrifugation
- Literature
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
- Teaching methods
- Study materials are available in the IS.
- Assessment methods
- Students would independently manage the entered tasks and correctly interpret the results in laboratory protocols.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi4020c Molecular biology - laboratory practice
(C1600 || C1080 || C1620 || C1650 || C2100 || C2500 || C2740 || C2480 || Bi9410 || souhlas) && (Bi4020 || NOW(Bi4020)) && !NOWANY(Bi4010c,Bi4035) && !Bi4010c && !Bi4035 - Bi6400c Methods of Molecular Biology - practice
(Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020 || Bi4035) && NOW(Bi6400) && !B6405 - Bi9310 Real-time polymerase chain reaction
Bi4035 || Bi4020c - Bi9310c Real-time polymerase chain reaction – practice
(Bi4035 || Bi4020c) && NOW(Bi9310)
- Bi4020c Molecular biology - laboratory practice
- Enrolment Statistics (Spring 2017, recent)
- Permalink: https://is.muni.cz/course/sci/spring2017/Bi4035