C8302 Proteomics - practice

Faculty of Science
Spring 2017
Extent and Intensity
0/3. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
RNDr. Hana Konečná (seminar tutor)
Mgr. Gabriela Lochmanová, Ph.D. (assistant)
Ing. Blanka Pekárová, Ph.D. (seminar tutor)
Mgr. Radka Dopitová, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (assistant)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Bc. Martina Válková (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable of Seminar Groups
C8302/Beh_01: No timetable has been entered into IS.
C8302/Beh_02: No timetable has been entered into IS.
Prerequisites (in Czech)
C8202 Proteomics || CG080 Genomics and proteomics ||NOW( C8202 Proteomics )||NOW( CG080 Genomics and proteomics )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 0/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
Course objectives
After completion of the course, the student will understand pronciples of the basic proteomics approaches, i.e. structural and functional study of proteins using recombinant proteins technology, protein separation using different techniques, predominantly two-dimensional electrophoresis and liquid chromatography and basic techniques of protein mass spectrometry. Students will be able to practically apply those approaches during solution of recent problems of both basic and applied research.
Syllabus
  • Two-dimensional electrophoresis of proteins
  • Mass spectrometry of proteins (MALDI-TOF, LC-MS/MS, sample preparation and analysis, protein identification)
  • Preparation, isolation and sequencing of recombinant DNA
  • Expression of recombinant DNA in E.coli
  • Single and multistep purification of recombinant proteins
  • Purity determination and analysis of recombinant proteins
  • Western bloting
Literature
  • THIELLEMENT, Hervé. Plant proteomics : methods and protocols. Totowa, N.J.: Humana Press, 2007, xiii, 399. ISBN 9781597452274. info
  • LIEBLER, Daniel C. Introduction to proteomics : tools for the new biology. Totowa, NJ: Humana Press, 2002, ix, 198 s. ISBN 0-89603-991-9. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of recent proteomics.
Assessment methods
The course will and up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2017, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2017/C8302