FA603 Electron microscopy in biology

Faculty of Science
spring 2018
Extent and Intensity
1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
Ing. Jana Nebesářová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: prof. Dr. Jiří Kozelka, PhD.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Timetable
Thu 10:00–10:50 F2 6/2012
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
  • Applied Physics (programme PřF, B-AF, specialization Medical Physics)
  • Biophysics (programme PřF, B-FY) (2)
  • Biophysics (programme PřF, N-FY, specialization Aplikovaná biofyzika) (2)
  • Biophysics (programme PřF, N-FY, specialization Molekulární biofyzika) (2)
Course objectives
The course introduces basic physical principles of transmission and scanning electron microscopy, and preparation methods of biological objects for electron microscopy.
Syllabus
  • Physical principles of electron microscopy and physical properties of accelerated electrons, resolution, magnification
  • Transmission electron microscope, electromagnetic lenses and defects, construction of the microscope
  • Preparation of specimens for TEM, physical methods, cryomethods, microwaves
  • Preparation of specimens for TEM, chemical methods, fixation, dehydration, embedding
  • Preparation of ultra-light cuts, ultramicrotome, knives, contrasting, underlying films
  • Scanning electron microscopy, construction of the microscope
  • Preparation of specimens for SEM, drying, coating
  • Electron microscopy cytology
  • Artifacts caused by TEM and SEM preparation, by microscope, photographic process
  • Microscopic images, digital and classical photography
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017.
  • Enrolment Statistics (recent)
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