C6790 Mass Spectrometry

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Pavel Brož, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Pavel Brož, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Mon 9:00–10:50 C12/311
Prerequisites
Physical Chemistry I. and II., Chemical structure, Quantum chemistry I. (C3140,C4020, C5020, C4060)
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 28 fields of study the course is directly associated with, display
Course objectives
After finishing the course, student will know principles and development of mass spectrometry, methods of ionisation and desorption of molecules: electron ionisation, methods of chemical ionisation, field ionisation and field desorption, laser ionisation, MALDI, and fast atom bombardment.
Student will understand principles of separation of ions in mass spectrometry using sector mass spectrometers, detection of metastable ions, and separation of ions by using of dynamical mass spectrometers.
Student will understand also joining of chromatographic methods with mass spectrometers: GC-MS, LC-MS, thermospray, electrospray, and he will learn to analyse surfaces of solid substances: SIMS, and principles of trace analysis: SS-MS, ICP-MS. Student will be also familiar with the direct inlet probe, membrane inlet and high temperature mass spectrometry. He will know to search spectra in libraries.
The aim of course is to give students basic information on mass spectrometry, which make it possible to use the mass spectrometry in praxis.
Syllabus
  • 1. Mass spectrometry as spectroscopic method. Physico-chemical and analytical information in mass spectrometry. Base peak and molecular peak. 2. Electron ionisation. Ion source, critical potentials, fragmentation. Statistical theory of fragmentation. Field ionisation. 3. Main types of reactions of monomolecular decay of ions of organic compounds. Cleavage of bonds. Rearangements. 4. Methods of chemical ionisation (CI a NCI). Ionisation at atmospheric pressure (API a APCI). Fragmentation of "quasimolecular" ions. Condensation reaction. 5. Methods of desorption: electric field, laser, plasma 252Cf, fast atoms and ions. 6. Mass analysers I. Introduction to vacuum technique. Sector mass spectrometers. Double focusing instruments. Detection of metastable ions. 7. Mass analysers II. Dynamic analysers. Quadrupole mass spectrometer. Monopole analyser. Ion trap. Ion cyclotron resonance. Time-of-flight mass spectrometer. Detectors of ions. 8. Coupling of chromatographic methods with mass spectrometry I. Gas chromatography - GC/MS, SFC/MS, TLC/MS. 9. Coupling of chromatographic methods with mass spectrometry II. Liquid chromatography - LC/MS: thermospray, electrospray, particle beam. 10.Tandem mass spectrometry. Collision activation. Arrangement of sector mass spectrometers. Ion trap as tandem. Interpretation of mass spectra. 11.Quantitative mass spectrometry of organic compounds. Types of spectra. Isotopic peaks. Dilution analysis. 12.Mass spectrometry in inorganic chemistry. Analysis of surfaces of solids - SIMS. Trace analysis - SSMS, ICP-MS. 13.High temperature mass spectrometry. Analysis of equilibrium vapour pressures. Thermodynamic data from mass spectrometry. Thermal desorption mass spectrometry (DIP). 14.Special mass spectrometries: membrane introduction (MIMS), electrochemical introduction (DEMS). Good laboratory praxis. Mass spectra libraries.Present state of mass spectrometry instrumentation.
Literature
  • BARKER, James. Mass spectrometry : analytical chemistry by open learning. Edited by David J. Ando. 2nd ed. Chichester: John Wiley & Sons, 1998, xxii, 509. ISBN 0-471-96764-5. info
Teaching methods
Theoretical preparation in lectures containing many practical examples.
Assessment methods
Oral exam. Analyses of 2 mass spectra.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2019, recent)
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