Bi5220c Immunology - practical course

Faculty of Science
Spring 2020
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Alena Žákovská, Ph.D. (seminar tutor)
MVDr. Mgr. Monika Dušková, Ph.D. (seminar tutor)
Mgr. Pavel Dobeš, Ph.D. (seminar tutor)
Mgr. Jana Hurychová, Ph.D. (seminar tutor)
doc. Mgr. Lukáš Kubala, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Lukáš Kubala, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Žákovská, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi5220c/01: Wed 12:00–13:50 D36/215, A. Žákovská
Bi5220c/03: Tue 16:00–17:50 D36/215, M. Dušková
Bi5220c/04: Wed 10:00–11:50 D36/215, P. Dobeš
Bi5220c/05: Tue 11:00–12:50 D36/215, J. Hurychová
Prerequisites (in Czech)
Bi5220 Immunology || Bi5220en Immunology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The exercise follows the basic lecture on Immunology. The main focus is theoretical and practical acquaintance with the methods used in immunological research and clinical laboratory practice. Taught methodologies belong to basic immunological methods. Emphasis is placed on students' own practical activities and the acquisition of good laboratory practice.
Learning outcomes
Upon completion of the course the student will be able to:
- describe the structure and function of immune organs and cells
- know the principles of basic immunological methods and will be able to use them in research
- describe the advantages and disadvantages of immunological methods and apply them appropriately
- perform a basic blood sample analysis
- perform and evaluate measurements of major immune parameters
Syllabus
  • 1. Histology of immune organs 2. Celular immunity and cells mediating the immune response 3. Immuno-diffusion: determination of lysozyme activity by radial diffusion 4. Phagocytic activity: measurement of oxidative burst by chemiluminometric method 5. Isolation of immune cells: separation of lymphocytes by gradient sedimentation 6. Hemagglutination methods: determination of blood groups 7. Invertebrate immunity: determination of phenoloxidase activity 8. Antioxidants: measurement of total antioxidant activity using TRAP method 9. Determination of antibodies: immuno-diffusion and turbidimetry 10. Chemiluminescent method for measurement of complement activity 11. Immunoelectrophoresis: detection of proteins in blood serum
Literature
  • Vít Hubinka, Monika Dušková, Alena Žákovská, Karel Vostal, Pavel Dobeš. Praktická cvičení z imunologie. Skriptum, MU, Brno, 2010.
  • HOŘEJŠÍ, Václav and Jiřina BARTŮŇKOVÁ. Základy imunologie. Vyd. 1. V Praze: Triton, 1998, 219 s. ISBN 808587573X. info
Teaching methods
practical course
Assessment methods
Attendance in the course and protocols from all lessons are compulsory; final exam in written form consisting of 20 questions minimum pass level 75 %.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2020, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2020/Bi5220c