C7943 Bioanalytics II - Laboratory medicine

Přírodovědecká fakulta
jaro 2021
Rozsah
2/0/0. 2 kr. (plus ukončení). Doporučované ukončení: zk. Jiná možná ukončení: k.
Vyučující
doc. Mgr. Jan Havliš, Dr. (přednášející)
Garance
doc. Mgr. Jan Havliš, Dr.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Dodavatelské pracoviště: Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Předpoklady
basic knowledge of biochemistry, of analytical, organic and inorganic chemistry are pre-requisited.
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
předmět má 9 mateřských oborů, zobrazit
Cíle předmětu
main objective of the course is to introduce the bioanalytics to its main field - laboratory medicine and diagnostics - to the students.
Výstupy z učení
at the end of the course students should be able:
to define relation between bioanalysis and illness diagnosis
to evaluate and apply respective bioanalytical approaches starting with method selection, through analyte determination to results presentation
to understand principles of basic methodical approaches and common instrumental techniques.
Osnova
  • 1. – introduction, samples – state specific analyte, sample types and their properties, preanalytical phase
  • 2. – classic instrumentation – analysers with liquid and solid reagencies; organisation, integration and miniaturisation of analyses; analytical sets
  • 3. – clinical metobolomics – metabolite, metabolome, metabolomics/metabonomics; basic concepts, importance, goals and demands; analytical platforms, data
  • 4. – advanced instrumentation – chromatography, electrophoresis, mass spectrometry
  • 5. – immunoanalysis – antigen, antibody; methods of Ag-Ab complex detection, immunodiagnostics, immunohistochemistry, immunocytochemistry
  • 6. – analysis of proteins – non-specific and specific, protein chips, enzyme analysis, enzyme as analyte and enzyme as analytical agent; role of buffers in bioanalysis
  • 7. – colourness – physical phenomenon and its utilisation in bioanalysis; medical microbiology, cultivation, biochemical and proteomic evidence, sensitivity to antibiotics
  • 8. – analysis of DNA – genetics and epigenetics in clinical praxis; NA preparation approaches, cytogenetics, identification of known and unknown sequence, methylation analysis
  • 9. – quality in clinical analysis – check and control; calibration and reference materials, operative quality control, validation, GLP
  • 10. – analytical methods – choice and optimisation; analytical method characteristics, choice of analytical methods
  • 11. – analytic result – its presentation; interpretation, uncertainty sources, variability; case study – development of a method for clinical diagnostics
  • 12. – selected analytes – inorganic, organic and macromolecular bioanalytes
Literatura
  • Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood - David E. Bruns - Barbara G. Sawye. 6th ed. St. Louis: Saunders/Elsevier, 2008, xx, 952. ISBN 9780721638652. info
  • Clinical laboratory diagnostics : use and assessment of clinical laboratory results. Edited by Lothar Thomas. 1st ed. Frankfurt am Main: TH-Books, 1998, 1527 s. ISBN 3980521540. info
Výukové metody
the lecture is based on ppt presentation and its explication. presentation it-self will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because of limited availability of textbooks in english covering certain parts of the subject. subject will be eventually lectured if the number of enrolled students exceeds number 5, otherwise students will be given the chance to pass the subject without lecturing combining study materials and on-demand tutorial (at max 2x2h per semester; contact the lecturer to set up the schedule).
Metody hodnocení
oral examination; students are required to understand and be familiar with the principles and its applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
Vyučovací jazyk
Angličtina
Další komentáře
Studijní materiály
Předmět je dovoleno ukončit i mimo zkouškové období.
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
Předmět je zařazen také v obdobích jaro 2013, jaro 2014, jaro 2015, jaro 2016, jaro 2017, jaro 2018, jaro 2019, jaro 2020, jaro 2022, jaro 2023, jaro 2024, jaro 2025.