Bi0124 Forensic genetics

Faculty of Science
Spring 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Eva Drozdová, Ph.D. (lecturer)
Mgr. Hana Svobodová (lecturer)
Guaranteed by
doc. RNDr. Eva Drozdová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Eva Drozdová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Thu 18:00–19:50 B11/305
Prerequisites (in Czech)
Bi5130 Human aDNA studies
Předpoklady studenta pro absolvování předmětu: minimálně absolvovaný předmět z obecné genetiky, molekulární biologie, genetiky člověka, případně biochemie.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
This course introduce students with basic principles of identification based on DNA analysis, intrpretation and evaluation of recived data in the socpe of problems of law and ethics.
Learning outcomes
At the end of the course will be students able to understand basic principles of identification by DNA analysis, to interprete and evaluate recived data in the socpe of prolbematic of law and ethic.
Syllabus
  • Forensic genetics as a science
  • History of forensic genetics or how did it actually begin? - Alec Jeffreys and DNA fingerprinting - Colin Pitchfork - 1st case of using DNA analysis in the world's forensic science - Milan Lubas - 1st case of DNA analysis for forensic purposes in Bohemia, Moravia and Slovakia - What was before DNA profiling?
  • Introduction to genetics. Structure of nuclear and mitochondrial DNA, method of writing genetic information - chromosome, gene, locus, DNA marker - polymorphism, mutations; homozygotes, heterozygotes - heredity of genotypes in genotypes, hemizygos, haplotype - a new one - "free" DNA.
  • Biological traces and biological material - tissue origin, species origin, and origin of biological material - usability of biological material for forensic genetic analysis - handling of biological traces - properties and behavior of the biological material in trace - evidence potential of trace
  • Principles of forensic genetic analysis - individuality and identification - determination of kinship - management of biological traces - prediction of biogeographic origin and phenotypic characteristics of a person
  • Forensic genetic analysis technology - DNA extraction - DNA quantification and DNA characterization - DNA amplification - Electrophoresis - STR loci analysis, HVR analysis mt DNA, SNP loci analysis - Degraded DNA, Mixed samples, LCN - Stochastic phenomena - etc.
  • Interpretation of forensic genetic data - general steps and rules of data interpretation - data reliability, the technical quality of data - quality in the laboratory - the informative value of data - statistical evaluation of data - sources of errors in the forensic genetic expertise
  • "Non-human" Forensic Analysis - Forensic Analysis of Animal DNA - Forensic Analysis of Plant DNA - Forensic Analysis of Microbial DNA
  • Preservation of genetic information sources - banks (archives, deposits) of biological material - banks of DNA isolates - DNA databases
  • Legislation and ethics of DNA analysis
Literature
    recommended literature
  • Butler, J. M. (2010): Fundamentals of Forensic DNA Typing. Elsevier Inc. Butler, J. M. (2011): Advanced Topics in Forensic DNA Typing: Methodology. Elsevier Inc. Šimková, H.: Breviář forenzní genetiky (Forenzní analýza DNA v otázkách a odpovědích).
Teaching methods
theoretical lectures, excursion in the laboratory
Assessment methods
essay writing on given topic, oral exam
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2022/Bi0124