Bi7031cen Microbiology – Practical Course

Faculty of Science
Spring 2022
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Ivan Kushkevych, Ph.D. (seminar tutor)
Guaranteed by
doc. Ivan Kushkevych, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 16:00–19:50 E25/111
Prerequisites (in Czech)
(! Bi5710c Microbiology - pr. ) && (! Bi4090c General microbiology - pr. ) && (! Bi2060c Basic microbiology - practice ) && !NOWANY( Bi5710c Microbiology - pr. , Bi4090c General microbiology - pr. , Bi2060c Basic microbiology - practice )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
The goal of practical exercises in general microbiology is to teach students basic methods of work in microbiological laboratory (methods of sterile work, isolation, seeding and cultivation of microorganisms, preparation of microscopic preparations and work with microscope, determination of number of bacterial cells in different types of samples, yeasts and microscopic fungi).
Learning outcomes
Learning outcomes of the course unit will be practical knowledge and ability of students to use microbiological methods in the practice.
Syllabus
  • Introduction, organization of exercises, safety of work in microbiological laboratory. Preparation of nutrient media and their sterilization. Isolation, seeding and cultivation of microorganisms. Microscopic observation of bacterial cells (native and fixed slides, Gram staining, work with microscope and image analysis). Determination of the total number of viable of bacterial cells by a plate method. Bacteria in water: basic microbiological analysis of the water. Determination of concentration of antibiotics by microbiological methods. Determination of bacterial sensitivity to antibiotics. Basic identification of bacteria, biochemical and physiological tests. Yeast, native slide, vital test, slide culture, cell size measurement. Detection and isolation of some soil microorganisms (cellulolytic activity, Azotobacter, Clostridium), microscopic fiber fungi. Assessment of the presence of microorganisms in the soil. Negative staining. Staining of Azotobacter sp. Observation of bacterial spore. Microscopic fibrous fungi: Credit final test.
Literature
    recommended literature
  • Teacher’ presentations
  • Kerr, J. (2004). Manual of clinical microbiology.
  • Arora, B., & Arora, D. R. (2007). Practical microbiology. CBS Publishers & Distributors.
  • Roberts, D., & Greenwood, M. (2008). Practical food microbiology. John Wiley & Sons.
  • Goldman, E., & Green, L. H. (2015). Practical handbook of microbiology. CRC press.
Teaching methods
At the beginning of each practical class, the students are acquainted with the content of the class in the form of explanation and demonstrations, then perform the assigned practical task. They draw up a protocol from each class.
Assessment methods
Active participation in practical classes, protocols, slide preparation and written test.
Language of instruction
English
Further comments (probably available only in Czech)
The course is taught each semester.
Information on course enrolment limitations: Minimalní počet studentů - 5
The course is also listed under the following terms Spring 2019, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2022/Bi7031cen