C7858 Elektromigrační metody

Faculty of Science
Spring 2023
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
Mgr. Anna Týčová, Ph.D. (lecturer)
Mgr. Jan Přikryl, Ph.D. (lecturer)
Mgr. Filip Duša, Ph.D. (lecturer)
Ing. Mgr. Roman Řemínek, Ph.D. (lecturer)
Mgr. Ivona Voráčová, Ph.D. (lecturer)
Ing. Markéta Laštovičková, Ph.D. (lecturer)
doc. RNDr. Petr Kubáň, Ph.D. (lecturer)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jan Preisler, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Thu 12:00–13:50 C14/207
Prerequisites
The elementar knowledge of Analytical Chemistry.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
In these classes, we will systematically introduce the main analytical methods using the separation of analytes in the electric field. Special attention will be devoted to the acquiring of the principles of the discussed methods and their employment in analytical praxis. The lectures will be held by highly experienced researchers with rich practical experiences.
Learning outcomes
A student knows the principles of the behaviour of analytes in the electric field and knows the main factors, which influence this behaviour. The student understands these principles in the context of discussed analytical methods.
Syllabus
  • 1. Introduction into electromigration methods. (Anna Týčová) Movement of molecules in electric field, electroosmotic flow, electrolysis, factors influencing the separation performance.
  • 2. Capillary zone electrophoresis. (Anna Týčová) Characteristics and principles, set-up, treating the capillaries, online concentration techniques, commercially available instrumentations, nonaqueous electrophoresis.
  • 3. Loading. Data collection and simulation of electrophoretic processes. Portable set-ups. (Petr Kubáň) Loading in capillary electrophoresis and special applications related to sample loading, on-line loading. Softwares for simulation of electrophoretic processes and data collection. Portable devices and their construction and application.
  • 4. On-column detections. (Jan Přikryl) The overview of theoretical and practical information about conductivity and optical (UV, VIS, fluorescence) detectors for sensing from a capillary. Multiplexed set-ups for CE-UV.
  • 5. Off-column detection. Electrophoresis in gaseous state. (Anna Týčová) Set-ups for coupling capillary methods to Raman and mass spectrometry. Electrochemical detection. Introduction in ion mobility spectrometry (IMS) and gas-phase electrophoretic molecular mobility analysis (GEMMA) and their application in practical examples.
  • 6. Electromigration techniques using selective interaction. Electrical field flow fractionation. (Jan Přikryl) The use of the chromatographic principle in electromigration methods, electrochromatography, micellar electrokinetic chromatography, affinity capillary electrophoresis, chiral separations in capillary electrophoresis. Principle and applications of electrical field flow fractionation (EFFF).
  • 7. Isotachophoresis. (Ivona Voráčová) Specifics of separation via isotachophoresis, instrumentation, coupling isotachophoresis to other separation methods, special types of isotachophoresis, applications.
  • 8. Isoelectric focusing. (Filip Duša) Characteristics and principles, isoelectric point, ampholytes and their anchoring in the separation environment, sets-ups for isoelectric focusing, applications.
  • 9. Gel electrophoresis. (Markéta Laštovičková) Separation on carriers, types of gels and their treating after the separation, special types of gel electrophoresis, combined electrophoretic methods, practical aspects of gel electrophoresis of proteins.
  • 10. Miniaturization I. (Anna Týčová) Practical aspects of miniaturization, clean laboratories. General principles of fabrication of glass microfluidic devices, dry and wet etching of glass, photolithography, examples of successful designs. Free-flow electrophoresis. Dielectrophoresis.
  • 11. Miniaturization II. (Jan Přikryl) Fabrication of plastic-based microfluidic devices, PDMS microfluidic devices, 3D printing, manufacturing of plastic material via micromachinery and laser beam, examples of successful designs.
  • 12. Validation of electromigration methods I. (Roman Řemínek) Importance of validation, validation parameters. The process of validation on examples of electromigration methods, specific aspects in the validation of electromigration methods. Industrial applications of electromigration techniques.
  • 13. Final information (Anna Týčová) Summary of the key information on the electromigration methods. Evaluation of the experimental records. Simple calculations related to electromigration methods.
Literature
    recommended literature
  • Electromigration techniques : theory and practice. Edited by Boguslaw Buszewski - Ewelina Dziubakiewicz - Michal Szumski. Heidelberg: Springer, 2013, xxi, 357. ISBN 9783642350429. info
    not specified
  • ČÁSLAVSKÝ, Josef and Jiří Georg Kamil ŠEVČÍK. Organická analýza. 1. vydání. Český Těšín: 2 Theta, 2022, 442 stran. ISBN 9788088279174. info
  • Gel electrophoresis of proteins : a practical approach. Edited by B. D. Hames - D. Rickwood. 2nd ed. Oxford: Oxford University Press, 1990, xviii, 383. ISBN 0199630755. info
Assessment methods
For passing the course, a written test with at least 50% of correct answers is required. The test is composed of open-ended questions.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Teacher's information
http://www.iach.cz/cs/
The lectures are given by respected scientists specialized in electromigration methods. All of them are employees of Institute of Analytical Chemistry of the CAS (IACH), where electromigration separations belong to well-established tools used for the research.

Therein, the demonstration of some discussed methods is possible upon request.

The course is also listed under the following terms Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/C7858