Bi1130c Methods in developmental biology - practical course

Faculty of Science
Spring 2024
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jakub Harnoš, Ph.D. (seminar tutor)
Mgr. Marek Hampl, Ph.D. (seminar tutor)
doc. RNDr. Marcela Buchtová, Ph.D. (seminar tutor)
Mgr. Pavel Dobeš, Ph.D. (seminar tutor)
Mgr. Jana Hurychová (seminar tutor)
RNDr. Petra Kolísková, Ph.D. (seminar tutor)
Mgr. Nikodém Zezula (seminar tutor)
Guaranteed by
doc. RNDr. Marcela Buchtová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Marcela Buchtová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 2. to Sun 26. 5. Fri 10:00–11:50 D36/215
Prerequisites (in Czech)
Bi1130 Methods in dev. biol. || NOW( Bi1130 Methods in dev. biol. )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, the students will get an overview of the most common experimental animal models used in Experimental Embryology and practical skills for their usage in a variety of experimental approaches.
Learning outcomes
Students will be able to:
- design simple experiments while using methods of Experimental Embryology
- practically manage experiment on model species
- determine developmental stages on models
- describe significant microscopic structures in selected developmental stages in model species
Syllabus
  • 1. Introduction to the course - methodology of scientific work, experimental design, assignment of student projects
  • 2. Drosophila - work with embryos
  • 3. C. elegans - work with embryos
  • 4. Danio I - work with embryos
  • 5. Danio II - comparative microscopic embryology
  • 6. Xenopus - work with embryos, microinjection
  • 7. Chicken model I - eggs opening, embryo staging, application of morphogen to chicken embryo (implantation, injection)
  • 8. Chicken model II - collection and processing of embryological material, skeleton staining, examples of developmental disorders
  • 9. Chicken model III - comparative microscopic embryology
  • 10. Mouse model I - collection and processing of embryological material, the establishment of primary cell and tissue cultures, transfection with GFP plasmid
  • 11. Mouse model II - LUC assay, cryosections, in vivo imaging on primary cell cultures
  • 12. Student project presentations
  • 13. Student project presentations
Literature
  • GILBERT, Scott F. Developmental biology. 9th ed. Sunderland, Mass.: Sinauer Associates, 2010, xxi, 711. ISBN 9780878935642. info
Teaching methods
Teaching is based on practical exercises on model species in the laboratory. Moreover, students will discuss their projects, which will be designed by them and presented to classmates. These presentations will be prepared by them individually at home. Microscopic slides will be demonstrated first by the teacher and then students will work with samples independently.
Assessment methods
Evaluation will be based on student performance during the presentation of their project. Quality of written assay, presentation, and discussion will be evaluated.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
The course is also listed under the following terms Spring 2022, Spring 2023, Spring 2025.
  • Enrolment Statistics (recent)
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