C4230 Biochemistry - laboratory course

Faculty of Science
Spring 2024
Extent and Intensity
0/0/6. 6 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
Mgr. Lukáš Faltinek (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Taťána Bržezická (assistant)
Mgr. Šárka Kobzová (assistant)
Mgr. Eliška Macháčová (assistant)
Mgr. Michaela Pešková (assistant)
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
C3181 Biochemistry I && ( C4221 Introductory Biochemistry Laboratory || C1100 Introductory Chemistry Lab )
Completion of the course Biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The aim of the laboratory course is to study the basic properties of compounds involved in biochemical processes and methodological approaches to the study and analytical use of enzymes. The first part covers the qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part deals with enzyme kinetics and further study of enzyme properties.
Learning outcomes
The students will be able to: - perform basic laboratory operations in a biochemical laboratory - operate common laboratory instruments used in a biochemical laboratory - mathematically evaluate measured data - interpret simple results of their work
Syllabus
  • Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.
Literature
    recommended literature
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
Teaching methods
Laboratory course
Assessment methods
The first laboratory exercise is dedicated to the teaching of safety in the biochemistry laboratory. Students also write an entrance test, the successful completion of which is a condition for participation in the exercise. Before each subsequent exercise, a written test is given to check the student's preparation for the task. In all tests, including the placement test, the student must answer at least 50% of the questions correctly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught each semester.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.
Information on course enrolment limitations: V podzimním semestru se vypisuje pouze jedna skupina pro 12 studentů chemických oborů jako náhrada za JS
The course is also listed under the following terms Spring 2025.
  • Enrolment Statistics (Spring 2024, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2024/C4230