Bi6120c Plant Cell, Tissue and Organ Culture - practical course

Faculty of Science
Spring 2025
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. Hana Cempírková, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi6120c/01: Mon 17. 2. to Sat 24. 5. Wed 12:00–13:50 C13/222, H. Cempírková
Bi6120c/02: Mon 17. 2. to Sat 24. 5. Wed 14:00–15:50 C13/222, H. Cempírková
Prerequisites (in Czech)
NOW( Bi6120 Plant Tissue and Organ Culture )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Passing through this course, students are able to:
apply basic methods and techniques of plant cell, tissue and organ culture in vitro;
propose in vitro methods suitable for plant multiplication, breeding and production;
estimate efficiency of the selected method for specific plant material on the base of visible symptoms.
Learning outcomes
At the end of this course, students should be able to:
- work in sterile conditions of in vitro cultures;
- prepare culture media and sterilize them;
- create and maintain different in vitro cultures;
- transfer plants from in vitro to glasshouse.
Syllabus
  • 1. Conditions of in vitro Culture, Laboratory Design.
  • 2. Media Preparation and Sterilization.
  • 3. In vitro Vegetative Propagation: Micropropagation by Proliferation of Axillary Buds, Adventitious Shoot Proliferation; Initiation of Primary Culture, Subculture. Transfer to ex vitro conditions.
  • 4. Callus Culture: Callus Initiation and Maintenance, Subculture, Organogenesis from Callus.
  • 5. Meristem Culture: Apical and Axillary Meristem Isolation and Culture.
  • 6. Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos. 7. Somatic embryogenesis: induction of somatic embryos from different materials 8. Ploidy reduction: androgenesis: initiation of isolated anthers culture
  • 9. Cryopreservation: Alginate Encapsulation/Dehydration of isolated axillary potato meristems.
  • 10. Fungi Culture in vitro: Suspension Culture of Wood-Destroying Fungi.
  • 11. Indexing and Certification of in vitro Cultures
Literature
  • SMITH, Roberta H. Plant tissue culture : techniques and experiments. Third edition. Amsterdam: Elsevier, 2013, xvi, 188. ISBN 9780124159204. info
  • KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
  • PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
  • GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
  • In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
  • SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press, 2003, xxii, 346. ISBN 0199254680. info
  • Phytohormones in plant biotechnology and agriculture : proceedings of the NATO-Russia Workshop held in Moscow, 12-16 May 2002. Edited by Ivana Macháčková - Georgy A. Romanov. Dodrecht: Kluwer Academic Publishers, 2003, xiv, 265. ISBN 1402017235. info
  • Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons, 2006, xii, 303. ISBN 0470021810. info
Teaching methods
Practical course in laboratory of in vitro cultures.
Assessment methods
Protocols of experiments, credit.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
Teaching is limited by the maximum number of students in one seminar group due to the size of the laboratory and the availability of teaching equipment. It is strongly recommended that you register for the course to allow enrollment in the lab. Without prior registration, a student may be denied enrollment in the course due to insufficient lab capacity.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2026.
  • Enrolment Statistics (recent)
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