Bi7665c Cell and tissue cultures - practical training

Faculty of Science
Spring 2025
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
Mgr. Jiří Pacherník, Ph.D. (seminar tutor)
Katarzyna Anna Radaszkiewicz, Ph.D., M.Sc. (seminar tutor)
Guaranteed by
Mgr. Jiří Pacherník, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Jiří Pacherník, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites (in Czech)
Bi3030 Animal physiology && Bi4020 Molecular biology && Bi2080 Histology and organology && Bi7665 Cell and Tissue Cultures
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 8/12
fields of study / plans the course is directly associated with
Course objectives
At the end of the course the students are able to: Understand and explain principles of basic tissue culture techniques; Employ TC techniques in their own research; Routinely culture common cell lines; Characterize parameters of cell proliferation, differentiation, and apoptosis.
Learning outcomes
At the end of the course the students are able to: Understand and explain principles of basic tissue culture techniques; Employ TC techniques in their own research; Routinely culture common cell lines; Characterize parameters of cell proliferation, differentiation, and apoptosis.
Syllabus
  • 1. Routine in tissue culture. 2. Solution and material for tissue culture. 3. Induction of differentiation of various cell lines. 4. Cell proliferation and apoptosis and their analysis. 5. Biochemical determination of cellular functions.
Literature
  • Cell biology : A laboratory handbook. Vol. 3. Edited by Julio E. Celis. [1st ed.]. San Diego: Academic Press, 1994, xxix, 535. ISBN 0-12-164717-X. info
  • Cell biology :a laboratory handbook. Edited by J. E. Celis. [1st ed.]. San Diego: Academic Press, 1994, xxvii, 496. ISBN 0-12-164716-1. info
  • Cell biology :a laboratory handbook. Edited by J. E. Celis. [1st ed.]. San Diego: Academic Press, 1994, xxxvi, 683. ISBN 0-12-164715-3. info
Teaching methods
laboratory practise
Assessment methods
The examinations require candidates to demonstrate their ability to apply techniques in practice by using a case study scenario.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
General note: místo vyžadovaných předmětů (tzv. prerekvizit) lze uznat i absolvování obsahově shodných předmětů v odpovídajícím rozsahu na jiných fakultách.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
The course is also listed under the following terms Spring 2019, Spring 2020, Spring 2021, autumn 2021, Spring 2022, Autumn 2022, Spring 2023, Autumn 2023, Spring 2024, Autumn 2024.
  • Enrolment Statistics (recent)
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