Bi8920 Advanced Microscopic Methods

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Jakub Neradil, Ph.D. (lecturer)
RNDr. Jan Škoda, Ph.D. (lecturer)
Ing. Josef Jaroš, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Jakub Neradil, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jakub Neradil, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites (in Czech)
Bi1700 Cell Biology || Bi1700c Cell Biology - practice
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this course is to provide general overview of the principles and techniques of fluorescence and electron microscopy.
Learning outcomes
At the end of the course, students should be able to: explain the principles and application of main methods in fluorescence microscopy, describe the construction of fluorescence and confocal microscope, learn basic techniques of specimen preparation for fluorescence microscopy, use an appropriate method to detect specific cell structure or molecule.
Syllabus
  • Lectures: Introduction to fluorescence microscopy, principles of immunofluorescence detection, life cells imaging techniques, confocal microscopy, image analysis, FISH, SKY. Laboratory practice: preparing samples for fluorescence microscopy.
Literature
  • Life cell imaging: a laboratory manual, Edited by R.D. Goldman, D.L. Spector, Cold Spring Harbor Laboratory Press, 2005.
  • Cell imaging techniques: methods and protokols. Edited by D:J. Taatjes, B.T. Mossman. Humana Press, 2006.
Teaching methods
lectures, laboratory practice
Assessment methods
written test
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.
  • Enrolment Statistics (recent)
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