Bi8920c Fluorescence microscopy - practice

Faculty of Science
Spring 2025
Extent and Intensity
0/2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jakub Neradil, Ph.D. (seminar tutor)
RNDr. Jan Škoda, Ph.D. (seminar tutor)
RNDr. Petr Chlapek, DiS., Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Jakub Neradil, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Jakub Neradil, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites (in Czech)
Bi1700 Cell Biology || Bi1700c Cell Biology - practice && NOW( Bi8920 Advanced Microscopic Methods )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 72 student(s).
Current registration and enrolment status: enrolled: 0/72, only registered: 92/72
fields of study / plans the course is directly associated with
there are 13 fields of study the course is directly associated with, display
Course objectives
The aim of this course is to provide general overview of the principles and techniques of fluorescence and electron microscopy.
Learning outcomes
At the end of the course, students should be able to: explain the principles and application of main methods in fluorescence microscopy, describe the construction of fluorescence and confocal microscope, learn basic techniques of specimen preparation for fluorescence microscopy, use an appropriate method to detect specific cell structure or molecule.
Syllabus
  • Laboratory practice: preparing samples for fluorescence microscopy.
Literature
  • Electron microscopy : methods and protocols. Edited by John Kuo. Third edition. New York: Humana Press, 2014, xviii, 799. ISBN 9781493960736. info
  • Live cell imaging : a laboratory manual. Edited by Robert D. Goldman - Jason Swedlow - David L. Spector. 2nd ed. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2010, xvi, 736. ISBN 9780879698935. info
  • Cell imaging techniques : methods and protocols. Edited by Douglas J. Taatjes - Brooke T. Mossman. Totowa, N.J.: Humana Press, 2006, xiv, 490. ISBN 158829157X. info
Teaching methods
lectures, laboratory practice
Assessment methods
written test
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.
  • Enrolment Statistics (recent)
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