Methods of Protein Characterization

Protein characterization by biochemical methods

Lecturer:  Mgr. Josef Houser, Ph.D. 

email: josef.houser@ceitec.muni.cz

Syllabus:

  1. Biomolecular sample, quality analysis, sample requirements 

  2. Methods for analysis of biomolecules, complementarity of techniques

  3. Biomolecular interactions – methods, comparison, applications

  4. Optimization of experiment, global analysis approach

  5. The high-throughput approach in biomolecular interactions

Recommended literature:

  • M. Baker: 1,500 scientists lift the lid on reproducibility. Nature 533, 452-454, 2016

  • M. Bastos: Biocalorimetry - Foundations and Contemporary Approaches. CRC Press 2016

  • D.J.Houde, S.A.Berkowitz: Biophysical Characterization of Proteins in Developing Biopharmaceuticals. Elsevier, 2015

  • SPRpages:


Lecture - part 1:

Lecture part 2:

Presentations without the comments:

Biophysical techniques for HTS I
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Biophysical techniques for HTS II
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