aZLLM0421c Lékařská orální mikrobiologie I - cvičení

Lékařská fakulta
jaro 2025
Rozsah
0/2/0. 30. 1 kr. Ukončení: z.
Vyučováno kontaktně
Vyučující
MUDr. Ondřej Zahradníček (přednášející)
MUDr. Lenka Černohorská, Ph.D. (cvičící)
Mgr. Milada Dvořáčková, Ph.D., MBA (cvičící)
Mgr. Monika Dvořáková Heroldová, Ph.D. (cvičící)
Ing. Veronika Holá, Ph.D. (cvičící)
Mgr. Dominika Polaštík Kleknerová (cvičící)
Mgr. Katarína Rebrošová (cvičící)
prof. MUDr. Filip Růžička, Ph.D. (cvičící)
Mgr. Pavlína Urbanová (cvičící)
Mgr. Lukáš Vacek, Ph.D. (cvičící)
doc. MUDr. Vladana Woznicová, Ph.D. (cvičící)
Garance
prof. MUDr. Filip Růžička, Ph.D.
Mikrobiologický ústav – Společná pracoviště s Fakultní nemocnicí u sv. Anny v Brně – Lékařská fakulta
Kontaktní osoba: MUDr. Ondřej Zahradníček
Dodavatelské pracoviště: Mikrobiologický ústav – Společná pracoviště s Fakultní nemocnicí u sv. Anny v Brně – Lékařská fakulta
Předpoklady
aZLLC011p Med Chem, Dental Mat - lect && aZLBI0222p Biologie II - přednáška && aZLBF011p Lékařská fyzika a infor.-lec.
Students are supposed to have considerable knowledge of anatomy, histology, biology, biochemistry and physiology.
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
At the end of this course, the students are supposed to understand methods used in medical microbiology, using medical oral microbiology.
Výstupy z učení
At the end of this course, students should be able:
to prepare a wet mount, Gram-stained preparation, to observe the results in a microscope, to list other staining methods
to list culture media, their use, differences between them, to reinoculate a strain/specimen to a culture medium
to identify bacteria and yeasts by means of various methods of biochemical identification and other identification methods
to describe antibiotic susceptibility/resistance testing methods and to read their results
to describe animal experiment (only basically)
to read results of nucleic acid detection in microbiology (only basically)
to describe methods detecting antigens or antibodies, their use for antibody detection/antigen detection in specimen/antigen analysis of a strain; to read the results of these methods including titers, titer dynamics, detection of IgM/IgA/IgG; to interpret the results; to describe precipitation, agglutination, agglutination on carriers, complement-fixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will be able to describe the basis of medical virology
Osnova
  • S01 Safety in a laboratory, microbes and outer influences, decontamination methods
  • S02 Technique of work with loop, wet mount. Gram staining, demonstration of more staining methods. Demonstration of pictures of various organisms and structures in Gram staining.
  • S03 Bacterial culture, cultivation media
  • S04 Metabolism of bacteria, identification according to biochemical activity, modern identification methods
  • S05 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • S06 Molecular methods in microbiology
  • S07 Introduction to serology, agglutination and precipitation, titres, dynamics of titres, complement-fixing test, neutralisation
  • S08 Reactions with labeled components (IMF, RIA, ELISA, CLIA/CMIA) including immunoblotting and immunochromatographic reactions
  • S09 Medical virology I (influenza, SARS-CoV-2 and other respiratory viruses)
  • S10 Medical virology II (hepatitis, HIV, herpesviruses, measles, mumps and rubella)
  • S11 Medical bacteriology I (easily culturable bacteria)
  • S12 Medical bacteriology II (bacteria with difficult cultivation)
  • S13 Medical parasitology (basics)
  • S14 Medical mycology (basics)
  • S15 No practical sessions (only exceptionally individual substitutions)
Literatura
    povinná literatura
  • SAMARANAYKE, L.P. Essential Microbiology for Dentistry. 3rd Ed. Churchill Livingstone, 2006, 372 s. ISBN 978-0-323-04475-2. info
    doporučená literatura
  • MARSH, Philip. Oral Microbiology. Wright, 2002. ISBN 0-7236-1051-7. info
  • BJARNSHOLT, Thomas, Claus MOSER a Niels HØIBY. Biofilm Infections. Springer, 2011. ISBN 978-1-4419-6084-9. e-book info
    neurčeno
  • Book No 1 + lectures + all materials from practical sessions are basic for the examination. Other books are recommended
Výukové metody
practical training in lab
self-study with the use of e-learning materials
Metody hodnocení
Conditions for credits:
(1) absolving all practicals, with the following notes:
- two justified absences or absences approved by a teacher are allowed; students are obliged to show that they completed the knowledge (in laboratory reports and in their heads)
- more absences than two require a substitution
- not justified absences are not allowed (EXTRA PAYMENT IS REQUIRED FOR EVENTUAL SUBSTITUTION!!!)
(2) complete laboratory report (signature of a teacher is not necessary)
(3) successfully written final test (usually 10 multiple choice questions, each for one point; 7 points needed) (4) all ROPOT questionnaires completed successfully; if they are always fulfilled in time (prior to particular lab session), the student has a bonus of one point for the final test
Vyučovací jazyk
Angličtina
Informace učitele
Contact to teachers and other stuff can be found here:
https://www.muni.cz/en/about-us/organizational-structure/faculty-of-medicine/110113-deptof-microbiology


Organizing information can be found in the "Course related instructions" of Study materials of this subject in the IS.


More information can be also found on the wall poster in the practical hall.

Další komentáře
Poznámka k ukončení předmětu: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
Nachází se v prerekvizitách jiných předmětů
Předmět je zařazen také v obdobích jaro 2017, jaro 2018, jaro 2019, jaro 2020, jaro 2021, jaro 2022, jaro 2023, jaro 2024.