LF:aVLBI0222c Medical Biology II - pract.

aVLBI0222c Medical Biology II - practice

Faculty of Medicine
spring 2025

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

Guaranteed by

prof. RNDr. Ondřej Slabý, Ph.D.
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Ing. Lívia Eiselleová, Ph.D.
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine

Prerequisites (in Czech)

aVLBI0121c Biology I - pract.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (programme LF, M-GM)
General Medicine (eng.) (programme LF, M-VL)

Course objectives

The main objectives of the course are:
- learning the methods of risk evaluation for hereditary diseases of various molecular backgrounds in patients based on family anamneses
- understanding the basic principles of gene engineering
- understanding and practising selected molecular methods used for the examination of some cancers or hereditary and infectious diseases with regard to the selection of appropriate therapy

Learning outcomes

After completing the course, the student will be able to:
- evaluate the risk of hereditary diseases of various molecular backgrounds in patients based on assigned family anamneses of the diseases
- describes the basic principles of genetic prognosis and genetic counselling
- suggest and perform basic molecular-genetic diagnostic examinations of some hereditary, tumour and infectious diseases (PCR, restriction digest, gel electrophoresis) and interpret the obtained results
- explain selected basic techniques of gene engineering and their application in medicine, and, with knowledge of the used method, select the clones of cells with an inserted gene

Syllabus

Week 1: Genetic disorders - autosomal and gonosomal inheritance
Week 2: Extensions to basic genetics
Week 3: Genetic prognosis and genetic counselling
Week 4: Human population genetics
Week 5: Basic methods of molecular biology
Week 6: Control test 3 (knowledge from week 1 to week 5 of the spring semester - lectures, practices)
Week 7: Gene engineering – production of human proteins in bacterial cells
Week 8: Gene engineering – production of human proteins in bacterial cells
Week 9: Molecular diagnostics of human pathogenic bacteria
Week 10: Molecular diagnostics of human pathogenic bacteria
Week 11: Molecular diagnostics of cancer
Week 12: Molecular diagnostics of cancer
Week 13: Control test 4 (knowledge from week 7 to week 12 of the spring semester - lectures, practices)
Week 14: no practices, Re-sit test
Week 15: no practices, credit awarding

Literature

required literature

Protocols for practices are provided in electronic form in the Information System of the Masaryk University in the Study Materials of the course aVLBI0222c

Teaching methods

laboratory practice, class discussion

Assessment methods

Practical classes are mandatory.
Requirements for course completion: full attendance in the practices (1 absence, excused or unexcused, is allowed); all protocols completed (also for missed lessons) and checked and signed by appropriate teachers; successfully passed 2 written control tests during the semester (knowledge from lectures and practices, 20 questions in each test, 1 answer correct to be chosen from 4 possibilities, student needs to reach at least 24 points out of 40 from both of the tests). In case you do not pass control tests (you have less than 24 points), you write a re-sit of the control test (knowledge from lectures and practices of the entire semester, 20 questions, 1 answer correct to be chosen from 4 possibilities, student needs to reach at least 12 points). If you do not pass the re-sit of the control test, you have to write the final test (Credit test) based on knowledge of the entire semester's lectures and practices. The credit test comprises 20 questions: 10 test questions (multiple answers can be correct, and negative marking is obtained for incorrect answers) + 10 given terms for a written explanation. At least 10 points out of 20 are needed to pass.
In case of a student’s late arrival to the practice or poor activity at the practice, the student must write an essay length of 2 pages on a given topic. Same for the case of two absences in practice (excused or unexcused). Find the instructions for essay writing in the Instructions for the course in the Interactive syllabus in the IS. Bring the essay printed to the teacher who specified the topic (after making an appointment during consulting hours), and be ready to answer the teacher's questions regarding the topic.
In case of 3 absences (excused or unexcused), (or more than 3 absences all excused by the Office for Studies within 5 days from the beginning of the absence and introduced to the Information System), it is addressed individually. Course-unit credit is awarded only after the proof of the student's knowledge of the entire semester's topics (Credit test, see above).
The course-unit credit is not awarded in the case of 4 or more unexcused absences.

Language of instruction

English

Follow-Up Courses

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.
Information on the extent and intensity of the course: 45.

Teacher's information

https://is.muni.cz/auth/el/med/jaro2024/aVLBI0222c/index.qwarp
Interactive syllabus of the course:

https://is.muni.cz/auth/el/med/jaro2024/aVLBI0222c/index.qwarp

What to bring to each practice: printed protocol for the respective practice, your own writing materials. Download the protocol for each practice from the Study materials or Interactive syllabus in the IS, and study it in advance. Lab shoes are required, and lab coats and disposable gloves are not required, but lab coats are recommended for tasks with staining.

Substitution of practices: In exceptional cases (illness, appointment at the doctor’s or foreign policy, 1 or 2 times per semester as maximum), students can arrange a substitution with another group. To arrange a substitution, write an e-mail to the course contact person at least 24 hours before your planned substitution and specify the group (date and time) you would like to attend instead (see Interactive syllabus - Instructions for the course - Timetable). Substitution is only possible on the same teaching week. When the capacity of the group you intended to substitute with is fully used, the substitution does not need to be approved. Repeated substitution with other groups is not allowed.

Complete Biology instructions to be found at the following link:

https://is.muni.cz/auth/el/med/jaro2024/aVLBI0222c/op/Biology_practices_instructions.pdf?predmet=1482172

The course is also listed under the following terms Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024.

Enrolment Statistics (spring 2025, recent)
Permalink: https://is.muni.cz/course/med/spring2025/aVLBI0222c

LF:aVLBI0222c Medical Biology II - pract. - Course Information

aVLBI0222c Medical Biology II - practice

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