BLKLM051x Medical Microbiology - practice

Faculty of Medicine
Spring 2015
Extent and Intensity
0/0. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Ondřej Zahradníček (seminar tutor)
Michaela Gregorovičová (assistant)
Iva Holešovská (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Prerequisites
BLKLM0321c Medical Microbiology I - pr. || BLKLM0422c Medical Microbiology II - p.
The students are supposed to have completed subjects BLKLM0321 and BLKLM0322, including examination, if possible.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Specialist practice complements the practical part of education in Clinical Biochemistry, Clinical Hematology, Immunohematology and Transfusion medicine, Clinical Microbiology, Clinical Immunology, Clinical Genetics and Pathologic Anatomy – Histopathologic determination methods. Students become familiar with the organization of workflow processes and basic documentation of laboratory worksites and will perform select tasks and activities. Students will mostly come to contact with following topics: Material admission and its division into corresponding laboratories; Basics of media preparing for bacterial culture; Specimen processing for bacteriological examination (urine, throat swab, vaginal swab, sputum, pus, blood culture); Assessment of antibiotic susceptibility using diffusion disc test and microdillution method; Basic serological methods (Paul-Bunnell in micro-modifiactions OCH and AKE, complemetfixing test, ASO, latex-fixation test); Use of ELISA and PCR methods;
Syllabus
  • 1. Admission of material, its distribution into individual laboratories; expedition of results to individual clients
  • 2. Preparing culture media for bacteriological cultivation
  • 3. Bacteriology I (processing of individual specimens: throat/nasal swab, sputum, urine, pus, blood culture and other specimens)
  • 4. Bacteriology II (identification methods in bacteriology, antibiotic susceptibility testing methods – diffusion disc test, microdillution test)
  • 5. Gastrointestinal specimens processing and evaluation, basics of parasitology of gastrointetinal tract
  • 6. Laboratory of STI (processing of various specimens for bacteriology, mycology and diagnostics of Trichomonas, including dg. of gonorrhoea)
  • 7. Serology: clasical serological methods, modern serological methods
  • 8. Virology: clasical and modern methods used in serological virology, direct diagnostics of viruses; basics of PCR in microbiology
Literature
  • VOTAVA, Miroslav, Vlastimil OBDRŽÁLEK, Petr ONDROVČÍK, Filip RŮŽIČKA, Ondřej ZAHRADNÍČEK and Vladana WOZNICOVÁ. Lékařská mikrobiologie II. Přehled vyšetřovacích metod v lékařské mikrobiologii (Medical Microbiology II. Survey of Diagnostic Methods in Medical Microbiology). 1st ed. Brno: Masarykova univerzita. Lékařská fakulta, 2000, 309 pp. ISBN 80-210-2272-8. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology. 2nd revised ed.). 2. přepracované vydání. Brno: NEPTUN, 2005, 351 pp. ISBN 80-86850-00-5. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1st ed. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003, 495 pp. ISBN 80-902896-6-5. info
  • SEDLÁČEK, Ivo. Taxonomie prokaryot (Taxonomy of prokaryotes). 1st ed. Brno: Masarykova univerzita, 2007, 270 pp. 55-969C-2006 02/58 12Př. ISBN 80-210-4207-9. info
  • 1 = základní/basic, 2, 3, 4 doplňkové/supplementary.
Teaching methods
practical work in laboratories of Department of microbiology
Assessment methods
credit, condition for credit is to absolve the practice
Language of instruction
Czech
Further comments (probably available only in Czech)
General note: Všechny formy kontaktní výuky předmětu vyjma přednášek jsou povinné.
Information on the extent and intensity of the course: 40.
The course is also listed under the following terms Spring 2012, Spring 2013, Spring 2014, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020.
  • Enrolment Statistics (Spring 2015, recent)
  • Permalink: https://is.muni.cz/course/med/spring2015/BLKLM051x