MOEB1041c Experimental biophotonics - practice

Faculty of Medicine
spring 2020
Extent and Intensity
0/0/1. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Radim Chmelík, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Radim Chmelík, Ph.D.
Department of Optometry and Orthoptics – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Lenka Herníková
Supplier department: Department of Optometry and Orthoptics – Departments of Non-medical Branches – Faculty of Medicine
Timetable
Thu 11:00–11:50 VUT-A4/501
Prerequisites
Students are expected to have theoretical knowledge as from "Applied optics" course.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course provides an overview of modern experimental techniques used to determine structure, function and dynamics of macro and micro biological samples by means of electromagnetic radiation. Attention will be focused on non-invasive methods used for detection, diagnosis and treatment of diseases, including methods used in ophthalmology and optometry.
Learning outcomes
Deeper theoretical and chiefly practical knowledge of some modern techniques that use electromagnetic radiation to study structure, function and dynamics of biological samples and methods used to detect, diagnose and treat diseases.
Syllabus
  • 1. Diffraction by a grating. Measurement of spectra of different light sources using a spectrometer. Measurement of coherence length using an interferometer.
  • 2. Diffraction of a laser beam on 2D objects (apertures in opaque screen), calculation of parameters of the apertures from the diffraction image.
  • 3. LIBS and tomograph.
  • 4. Application of laser scalpel - microdissection technique.
  • 5. Practical demonstrations of the common methods enhancing contrast in an optical microscope.
  • 6. Confocal and fluorescence microscopy in biology. Application of holographic microscopy in study of alive mammalian cells.
Literature
  • J. B. Pawley: “Handbook of Biological Confocal Microscopy”, 2nd Edition, Plenum Press, 1995.
  • P. N. Prasad: “Introduction to Biophotonics”, John Wiley & Sons, Inc., 2003.
Teaching methods
laboratory practice, laboratory presentation and hands-on exercise, discussion
Assessment methods
The credit is awarded on the basis of active participation in teaching.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 15.
The course is also listed under the following terms Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (spring 2020, recent)
  • Permalink: https://is.muni.cz/course/med/spring2020/MOEB1041c