EMMT0411s Mikroskopické techniky - seminář

Lékařská fakulta
jaro 2022
Rozsah
0/2/0. 30. 4 kr. Ukončení: zk.
Vyučující
Ing. Josef Jaroš, Ph.D. (přednášející)
Garance
Ing. Josef Jaroš, Ph.D.
Ústav histologie a embryologie – Teoretická pracoviště – Lékařská fakulta
Dodavatelské pracoviště: Ústav histologie a embryologie – Teoretická pracoviště – Lékařská fakulta
Rozvrh
Čt 13:30–15:10 F01B1/126
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
The aim of this course is to explain principles of microscopy, methods of tissue processing and image analysis. Student will get the understanding about wide spectrum of microscopic techniques and their application. Student will learn methods of processing tissue samples – cells, tissues for individual microscopic techniques. Finally, student will be able to choose optimal method for detection of specific cell structures and molecules, able to explain and perform some of methods of processing of tissue samples for histological analysis with light microscopy.
Výstupy z učení
Student will:
- understand wide spectrum of microscopic techniques and their application.
- know methods of processing tissue samples – cells, tissues for individual microscopic techniques.
- be able to choose optimal method for detection of specific cell structures and molecules
- be able to explain and perform some of methods of processing of tissue samples for histological analysis with light microscopy.
Osnova
  • 1. Fundamentals of light microscopy – transmission light, dark field, contrast enhancement, stereomicroscopy, image acquisition
  • 2. Preparation of biological samples. Principles of histological staining.
  • 3. Fluorescence microscopy – epifluorescence, confocal, holographic, spectral microscopy, SPIM.
  • 4. Superresolution microscopy – SIM, STED, PALM, STORM.
  • 5. Live imaging of cells. Techniques. Staining of samples.
  • 6. Automated high content screening microscopy.
  • 7. Electron microscopy – principles, sample preparation, SEM, TEM, CryoTEM, SBF-SEM.
  • 8. Special microscopy – 2-photon, AFM, X-ray microscopy, microCT.
  • 9. Image processing and analysis.
Literatura
    doporučená literatura
  • Cell imaging techniques: methods and protocols. Edited by D:J. Taatjes, B.T. Mossman. Humana Press, 2006.
  • Theory and practice of histological techniques. Edited by John D. Bancroft - Marilyn Gamble. 5th ed. Edinburgh: Churchill Livingstone, 2003. xiii, 796. ISBN 0443064350.
Výukové metody
seminar
Metody hodnocení
written examination
Vyučovací jazyk
Angličtina
Další komentáře
Studijní materiály
Předmět je zařazen také v obdobích jaro 2023, jaro 2024, jaro 2025.