VLBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2022
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Zdenka Fohlerová, Ph.D. (seminar tutor)
Mgr. David Galuška (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
MUDr. Mgr. Pavel Šmak (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Jan Víteček, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Martina Hanousková (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
VLBC0422c/01: Fri 8:00–10:30 A16/212, J. Gregorová
VLBC0422c/02: Fri 8:00–10:30 A16/216, J. Víteček
VLBC0422c/03: Fri 8:00–10:30 A16/211, D. Galuška
VLBC0422c/04: Tue 14:00–16:30 A16/212, M. Králíková
VLBC0422c/05: Tue 14:00–16:30 A16/211, O. Peš
VLBC0422c/06: Tue 14:00–16:30 A16/216, J. Kos
VLBC0422c/07: Mon 11:00–13:30 A16/216, J. Kos
VLBC0422c/08: Mon 11:00–13:30 A16/211, Z. Fohlerová
VLBC0422c/09: Mon 11:00–13:30 A16/212, O. Peš
VLBC0422c/10: Mon 14:00–16:30 A16/216, J. Gregorová
VLBC0422c/11: Mon 14:00–16:30 A16/212, M. Králíková
VLBC0422c/12: Thu 14:00–16:30 A16/211, O. Peš
VLBC0422c/13: Fri 11:00–13:30 A16/211, J. Smutná
VLBC0422c/14: Fri 11:00–13:30 A16/216, J. Víteček
VLBC0422c/15: Fri 11:00–13:30 A16/212, J. Kos
VLBC0422c/16: Thu 14:00–16:30 A16/216, P. Šmak
VLBC0422c/17: Mon 14:00–16:30 A16/211, J. Kos
VLBC0422c/18: Thu 14:00–16:30 A16/212, J. Gregorová
VLBC0422c/19: Mon 8:00–10:30 A16/212, Z. Fohlerová
VLBC0422c/20: Mon 8:00–10:30 A16/211, D. Galuška
Prerequisites (in Czech)
VLBC0321s Biochemistry I - seminar && VLFY0321c Physiology I - practice && VLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to VLBC0421p. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Learning outcomes
After completing the course, the student will be able to:
- explain the methods of obtaining biological material for biochemical analyzes and know the rules of handling.
- describe the physiological values of the most important biochemical parameters.
- interpret the results of biochemical analyzes, discuss the significance of the findings.
- understand the principles of the most important biochemical-analytical methodologies.
- be oriented in basic laboratory tasks.
Syllabus
  • Organization of work in practical exercises. Handling with biological material.
  • Blood collection and processing.
  • Pipetting.
  • Communication of biochemical test results.
  • Determination of plasma glucose, determination of glycemia by personal glucometer. Oral glucose tolerance test (oGTT). Evidence of glucosuria and ketonuria. Determination of glycated hemoglobin.
  • Determination of total cholesterol in serum and blood. Determination of triacylglycerols in blood. Determination of HDL- and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. Determination of catalytic concentrations of aminotransferases in serum.
  • Determination of total serum bilirubin. Detection of bilirubin and urobilinogens in urine.
  • Determination of serum albumin.
  • Determination of total protein and serum albumin. Serum protein electrophoresis.
  • Detection and determination of C-reactive protein in serum and blood. Determination of ferritin in serum.
  • Objective and physical examination of urine.
  • Determination of creatinine in serum and urine. Determination of glomerular filtration from creatinine clearance. Calculation of fractional water resorption and excretion.
  • Proof and determination of proteinuria and albuminuria. Evidence of blood pigment in the urine. Detection of Bence-Jones protein in urine.
  • Determination of urea in serum and urine. Nitrogen balance.
  • Examination of urine with multifunctional diagnostic strips. Demonstration of drugs in urine. Detection of luteinizing hormone in urine.
  • Solubility of uric acid. Orientational chemical analysis of urinary stones.
  • Determination of uric acid in serum and urine.
  • Selected laboratory examinations of the stomach. Laboratory tests for pancreatic diseases Digestive tract occult bleeding test.
Literature
    required literature
  • Návody ke cvičením dostupné v IS MUNI.
Teaching methods
Laboratory practical classes
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all lessons,
  • elaboration of all protocols and insertion into the Homework vaults in IS MUNI,
  • successful completion of the credit test (at least 70% of points),
  • course-unit credit from ZC041.
    The detailed conditions are posted in IS MUNI (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu VLBC0422p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    https://is.muni.cz/auth/el/1411/jaro2022/VLBC0422c/
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.

    A white coat, slippers or sturdy shoes with overshoes, a respirator type FFP2, gloves, a calculator are required.

    Consultations are possible after a personal arrangement with the teacher.

    The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2023, spring 2024, spring 2025.
    • Enrolment Statistics (spring 2022, recent)
    • Permalink: https://is.muni.cz/course/med/spring2022/VLBC0422c