ZLHE0221c Histology and Embryology I - practice

Faculty of Medicine
spring 2022
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. MVDr. Martin Anger, CSc. (seminar tutor)
MUDr. Ivana Baltasová (seminar tutor)
MUDr. Kristína Brablecová (seminar tutor)
prof. MUDr. RNDr. Svatopluk Čech, DrSc. (seminar tutor)
MUDr. Jana Dumková, Ph.D. (seminar tutor)
doc. MVDr. Aleš Hampl, CSc. (seminar tutor)
Mgr. Hana Kotasová, Ph.D. (seminar tutor)
doc. RNDr. Irena Koutná, Ph.D. (seminar tutor)
MVDr. Anna Mac Gillavry Danylevska, Ph.D. (seminar tutor)
MUDr. Eva Mecová (seminar tutor)
MUDr. Volodymyr Porokh (seminar tutor)
Anas Rabata, M.Sc., Ph.D. (seminar tutor)
doc. MUDr. Miroslava Sedláčková, CSc. (seminar tutor)
MUDr. Veronika Sedláková, Ph.D. (seminar tutor)
MUDr. Yehonatan Solomonov (seminar tutor)
Mgr. Eva Švandová, Ph.D. (seminar tutor)
doc. RNDr. Petr Vaňhara, Ph.D. (seminar tutor)
Ing. Nicole Ludikovská (assistant)
Guaranteed by
doc. MVDr. Aleš Hampl, CSc.
Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
ZLHE0221c/21: Mon 14. 2. 10:30–12:10 F01B1/122, Mon 21. 2. 10:30–12:10 F01B1/122, Mon 28. 2. 10:30–12:10 F01B1/122, Mon 7. 3. 10:30–12:10 F01B1/122, Mon 14. 3. 10:30–12:10 F01B1/122, Mon 21. 3. 10:30–12:10 F01B1/122, Mon 28. 3. 10:30–12:10 F01B1/122, Mon 4. 4. 10:30–12:10 F01B1/122, Mon 11. 4. 10:30–12:10 F01B1/122, Mon 25. 4. 10:30–12:10 F01B1/122, Mon 2. 5. 10:30–12:10 F01B1/122, Mon 9. 5. 10:30–12:10 F01B1/122, Mon 16. 5. 10:30–12:10 F01B1/122
ZLHE0221c/22: Mon 14. 2. 10:30–12:10 F01B1/122, Mon 21. 2. 10:30–12:10 F01B1/122, Mon 28. 2. 10:30–12:10 F01B1/122, Mon 7. 3. 10:30–12:10 F01B1/122, Mon 14. 3. 10:30–12:10 F01B1/122, Mon 21. 3. 10:30–12:10 F01B1/122, Mon 28. 3. 10:30–12:10 F01B1/122, Mon 4. 4. 10:30–12:10 F01B1/122, Mon 11. 4. 10:30–12:10 F01B1/122, Mon 25. 4. 10:30–12:10 F01B1/122, Mon 2. 5. 10:30–12:10 F01B1/122, Mon 9. 5. 10:30–12:10 F01B1/122, Mon 16. 5. 10:30–12:10 F01B1/122
ZLHE0221c/23: Wed 16. 2. 10:30–12:10 F01B1/122, Wed 23. 2. 10:30–12:10 F01B1/122, Wed 2. 3. 10:30–12:10 F01B1/122, Wed 9. 3. 10:30–12:10 F01B1/122, Wed 16. 3. 10:30–12:10 F01B1/122, Wed 23. 3. 10:30–12:10 F01B1/122, Wed 30. 3. 10:30–12:10 F01B1/122, Wed 6. 4. 10:30–12:10 F01B1/122, Wed 13. 4. 10:30–12:10 F01B1/122, Wed 20. 4. 10:30–12:10 F01B1/122, Wed 27. 4. 10:30–12:10 F01B1/122, Wed 4. 5. 10:30–12:10 F01B1/122, Wed 11. 5. 10:30–12:10 F01B1/122, Wed 18. 5. 10:30–12:10 F01B1/122
ZLHE0221c/24: Wed 16. 2. 10:30–12:10 F01B1/122, Wed 23. 2. 10:30–12:10 F01B1/122, Wed 2. 3. 10:30–12:10 F01B1/122, Wed 9. 3. 10:30–12:10 F01B1/122, Wed 16. 3. 10:30–12:10 F01B1/122, Wed 23. 3. 10:30–12:10 F01B1/122, Wed 30. 3. 10:30–12:10 F01B1/122, Wed 6. 4. 10:30–12:10 F01B1/122, Wed 13. 4. 10:30–12:10 F01B1/122, Wed 20. 4. 10:30–12:10 F01B1/122, Wed 27. 4. 10:30–12:10 F01B1/122, Wed 4. 5. 10:30–12:10 F01B1/122, Wed 11. 5. 10:30–12:10 F01B1/122, Wed 18. 5. 10:30–12:10 F01B1/122
Prerequisites (in Czech)
ZLAN0131s Anatomy I - seminar || ZLAN0121s Anatomy I - seminar && ZC011 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course objectives
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy. 2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue. 3. Identify tissue types and organs of circulatory, lymphatic and respiratory systems in histologic slides. 4. Characterize microscopic structure of some organs of human body and explain correlations of tissues within organs. 5. Describe gamete development, explain principle of meiosis and regulation of gametogenesis. 6. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord. 7. Characterize main phases of prenatal development.
Learning outcomes
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy. 2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue. 3. Identify tissue types and organs of circulatory, lymphatic and respiratory systems in histologic slides. 4. Characterize microscopic structure of some organs of human body and explain correlations of tissues within organs. 5. Describe gamete development, explain principle of meiosis and regulation of gametogenesis. 6. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord. 7. Characterize main phases of prenatal development.
Syllabus
  • 1. Introduction, organization of practicals, teaching aids. Overview of histological methods. Tissue processing for light and electron microscopy - explanation and film. Basic histochemistry and immunocytochemistry.
  • 2. Cytology: Ultrastructure of the cell nucleus during interphase. Ultrastructure of cell organelles I (mitochondria, the Golgi apparatus, the endoplasmic reticulum, ribosomes, lysosomes).
  • 3. Ultrastructure of cell organelles II (peroxisomes, and the centriol). Cell inclusions. The arrangement of cell surfaces: apical, lateral, and basal ones. Intercellular junctions: adhering, occluding, and communicating ones.
  • 4. General embryology I. Aids: Set of embryological schemes and pictures (I).
  • 5. General embryology II. Aids: Set of embryological schemes and pictures (II).
  • 6. General histology: Use of the light microscope, common faults in microscopy. Results of basic staining methods in histology: Hematoxylin-eosine /HE/ (hepar), hematoxylin–eosine-saffron /HES/ (esophagus), AZAN (prostate), impregnation (cerebellum). Connective tissue proper - classification and examples. Slides: Funiculus umbilicalis, Esophagus, Posterior segment of the eye, Lien, Aorta.
  • 7. Supporting tissues: cartilage and bone. Histogenesis of the bone tissue (ossification). Slides: Trachea, Auricula, Elastic cartilage, Lamellar bone, Chondrogenic ossification.
  • 8. Epithelial tissue: epithelial membranes (covering epithelia) and glandular epithelium. Slides: Ren, Vesica fellea, Trachea, Esophagus, Ureter, Palpebra, Skin from the finger tip.
  • 9. Glandular epithelium. Skides: Intestinum tenue, Glandula parotis, Glandula submandibularis.
  • 10. Nerve tissue. Slides: Cortex cerebri, Cerebellum, Medulla spinalis, Ganglion spinale, Peripheral nerve, Motor end plate.
  • 11. Muscle tissue. Slides: Apex linguae, Cardiac muscle tissue Intestinum tenue.
  • 12. Blood cells: Preparation of blood smears and their staining. Cytology of formed elements of blood. Differential white cell count (dWCC) - average values, abnormities and terminology of dWCC. Slide: A smear of peripheral blood stained by Pappenheim's method. Credits.
Literature
    recommended literature
  • ČECH, Svatopluk, Drahomír HORKÝ, Irena LAUSCHOVÁ, Miroslava SEDLÁČKOVÁ and Jitka ŠŤASTNÁ. Histologická praktika a metody vyšetřování tkání a orgánů (Histological practicals and methods of investigation of tissues and organs). 1.dotisk 1. vydání. Brno: Vydavatelství MU Brno-Kraví hora, 2002, 162 pp. ISBN 80-210-1774-0. info
    not specified
  • MARTÍNEK, Jindřich and Zdeněk VACEK. Histologický atlas. 1. vyd. Praha: Grada, 2009, 134 s. ISBN 9788024723938. info
  • EROSCHENKO, Victor P. Di Fiore's atlas of histology with functional correlations. Edited by Mariano S. H. di. Fiore. 11th ed. Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins, 2008, xvii, 532. ISBN 9781608314928. info
Teaching methods
Content of this course includes study of slides and electron micrographs in microscopic hall as well as class discussion on the base of materials in the text book.
Assessment methods
Rules and conditions applicable for practical training and for examining in Histology and Embryology can be found on the website of Department of Histology and Embryology (http://www.med.muni.cz/histology/education) as Provision of the Head of the department on the rules and conditions applicable for practical training and for examining in histology and embryology/en.
Testing of knowledge: Student must prove sufficient level of knowledge by written test examination. Each student completes 4 partial tests during semester. Tests are evaluated by point for correct answer. More than half number of correct answers (points) is evaluated as passed "P". All of these tests must be successful. In case of failure (evaluation not passed "N"), only 1 resit is possible. There is condition 4 from 4 (ie. 4 P / 4 regular tests) or 4 from 5 (ie. 3 P / 4 regular tests + 1 P / 1 resit). If student does not fulfill this condition, credit test follows in the relevant exam period. This test covers all topics studied during semester. In case of failure in credit test, credit will not be given and student must enroll the course again.
Conditions for obtaining credit: 1. Attendance at all practical exercises (100% participation, all absences must be regularly excused (in IS) and substituted).
2. Successful completion of all tests.
3. Submission of all protocols (correctly completed forms of protocols signed by teacher).
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
Listed among pre-requisites of other courses
Teacher's information
http://www.med.muni.cz/histology/education
The course is also listed under the following terms Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (spring 2022, recent)
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