BLTS0411c Immunohaematology and Blood Banking - practice

Faculty of Medicine
Autumn 2009
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Eva Tesařová (lecturer), doc. MUDr. Milan Dastych, CSc., MBA (deputy)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Prerequisites (in Czech)
BLKH0311c Clinical Haematology - pract. || BLKH0411c Clinical Haematology - pract.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical part of education provides laboratory skills which provide easy formatting of school-leaver to immunohaematology laboratory teams or to transfusion service teams. The most important concentration is focused on adopt conscientious laboratory processes including responsibility for safety of all particular lab steps link to ethic consequences.; After course completing programme the student will be able: - do in praxis and control immunohaematology tests, inc. HLA tests - evaluate quality of analytic samples, - use laboratory IT systems, - know and use principles of good laboratory praxis and good manufacturing praxis, - partake in formation of SOPs.
Syllabus
  • Practical skills ; Techniques used in blood grouping, factors affecting antigen-antibody reactions, AB0 blood group testing, including weak blood groups and abnormalities, secretor status ; Techniques used in Rh system testing, including variant and weak D antigens, clinical importance in blood donors and patients and immunoprophylaxis ; Molecular blood group genotyping; Testing of blood group antibodies, tests to asses the potential significance of an antibody, decision making for transfusion ; Pretransfusion compatibility testing, special investigation in the workup of unexpected antibodies and blood group incompatibilities, special testing of patients with AIHA ; Immunohaematology testing of infant and newborn, blood group testing, direct antiglobulin test, management of transfusion compatibility, special requirements for intrauterine and neonatal transfusions ; Tests to evaluate suspected transfusion reaction ; Resolution of IgG and IgM antibodies ; HLA laboratory diagnostics, detection of I., II. Class of HLA antigens, HLA antibodies and its laboratory detection; Data process control on-line data transfer, record keeping ; Blood collection, testing and processing, blood donor selection, resp. blood donor management, storage and release of transfusion products ;
Literature
  • MATĚJKOVÁ, Eva, Darina OČADLÍKOVÁ, Jana SMEJKALOVÁ, Jana MUŽÍKOVÁ, Luděk RAIDA, Kateřina TOUŠOVSKÁ, Rita PACASOVÁ, Marie NENIČKOVÁ, Eva TESAŘOVÁ, Jaroslav ŠTĚRBA, Karel INDRÁK and Jaroslav MICHÁLEK. Selektivní deplece aloreaktivních T-lymfocytů a studium protinádorové aktivity specifických klonů T-lymfocytů u pacientů s leukémií (SELECTIVE DEPLETION OF ALLOREACTIVE T CELLS AND STUDY OF ANTI-TUMOR ACTIVITY OF SPECIFIC T CELL CLONES IN PATIENTS WITH LEUKEMIA). Klinická onkologie. Brno: ApS BRNO, spol. s r. o., 2008, vol. 21, No 3, p. 104-109, 5 pp. ISSN 0862-495X. URL info
  • HRABČÁKOVÁ, Viera, Petr ČOUPEK, Marie NENIČKOVÁ and Eva TESAŘOVÁ. Hladiny cytokinů a kostimulačních molekul (interleukinů IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, TNF-alfa, TGF-beta1, sCD23, sCD40L a receptoru pro IL-6) v plazmě zdravých dárců krve. (Plasma levels of cytokines and co-stimulatory molecules (interleukins IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, TNF-alpha, TGF-beta1, sCD23, sCD40L and receptor for IL-6) in healthy blood donors.). Transfuze a hematologie dnes. Praha: Česká lékařská společnost JEP, 2006, vol. 12, No 3, p. 166-172. ISSN 1213-5763. info
  • HRABČÁKOVÁ, Viera, Petr ČOUPEK, Marie NENIČKOVÁ and Eva TESAŘOVÁ. Hladiny cytokinů a kostimulačních molekul (interleukinů-2, 4, 5, 6, 8, 10, 13, TNF-alfa, TGF-beta1, sCD23, sCD40L a receptoru pro IL-6) v plazmě zdravých dárců krve. (Plasma levels of cytokines and co-stimulatory molecules (interleukin-2, 4, 5, 6, 8, 10, 13, TNF-alpha, TGF-beta1, sCD23, sCD40L and receptor for IL-6) in healthy blood donors.). In XIV. Slovensko-český hematologický a transfuziologický zjazd s medzinárodnou účasťou, 29.9.-2.10.2005, Štrbské Pleso. 2005. ISBN 80-968685-9-4. info
  • HRABČÁKOVÁ, Viera, Marie NENIČKOVÁ, Eva TESAŘOVÁ and Martin KLABUSAY. Hladiny interleukinů-2, 4, 5, 6, 8, 10, 13, TNF-alfa, TGF-beta1, sCD23, sCD40L a receptoru pro IL-6 v plazmě zdravých dárců krve. (Plasma levels of interleukin-2, 4, 5, 6, 8, 10, 13, TNF-alpha, TGF-beta1, sCD23, sCD40L and sIL-6 receptor in healthy blood donors.). In XIX. Olomoucké hematologické dny s mezinárodní účastí. 2005. ISBN 80-7346-051-3. info
Teaching methods
- self-reliant solution of analytic laboratory methods in teaching lab, - qualify accuracy of indication and interpretation of immunohaematology laboratory results, - continuous monitoring of student laboratory works and its corrections
Assessment methods
- active practical works participation, - evaluating of acquired knowledge and praxis skills, - fulfilling requirements.
Language of instruction
Czech
The course is also listed under the following terms Spring 2007, Spring 2008, Autumn 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022.
  • Enrolment Statistics (Autumn 2009, recent)
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