MBKB071p Clinical biochemistry II - lectures

Faculty of Medicine
autumn 2018
Extent and Intensity
3/0/0. 5 credit(s). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslava Beňovská, Ph.D. (lecturer)
MUDr. Zdeňka Čermáková, Ph.D. (lecturer)
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
Mgr. Jana Gottwaldová (lecturer)
MUDr. Ondřej Kyselák, Ph.D., EuSpLM (lecturer)
RNDr. Alena Mikušková (lecturer)
Ing. Martina Podborská, Ph.D. (lecturer)
prof. MUDr. Vladimír Soška, CSc. (lecturer)
Mgr. Jana Tomanová (lecturer)
MUDr. Hana Vinohradská (lecturer)
Mgr. Ondřej Wiewiorka, Ph.D. (lecturer)
Mgr. Miroslav Fiala (assistant)
Michaela Gregorovičová (assistant)
doc. RNDr. Josef Tomandl, Ph.D. (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Timetable
Mon 17. 9. 9:00–10:40 C05/107, Thu 20. 9. 11:00–12:40 C05/107, Mon 24. 9. 9:00–10:40 C05/107, Thu 27. 9. 11:00–12:40 C05/107, Mon 1. 10. 9:00–10:40 C05/107, Thu 4. 10. 11:00–12:40 C05/107, Mon 8. 10. 9:00–10:40 C05/107, Thu 11. 10. 11:00–12:40 C05/107, Mon 15. 10. 9:00–10:40 C05/107, Thu 18. 10. 11:00–12:40 C05/107, Mon 22. 10. 9:00–10:40 C05/107, Thu 25. 10. 11:00–12:40 C05/107, Mon 29. 10. 9:00–10:40 C05/107, Thu 1. 11. 11:00–12:40 C05/107, Mon 5. 11. 9:00–10:40 C05/107, Thu 8. 11. 11:00–12:40 C05/107, Mon 12. 11. 9:00–10:40 C05/107, Thu 15. 11. 11:00–12:40 C05/107, Mon 19. 11. 9:00–10:40 C05/107, Thu 22. 11. 11:00–12:40 C05/107, Mon 26. 11. 9:00–10:40 C05/107, Thu 29. 11. 11:00–12:40 C05/107, Mon 3. 12. 9:00–10:40 C05/107, Thu 6. 12. 11:00–12:40 C05/107
Prerequisites
bachelor's studies focused on laboratory medicine or natural sciences
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the theoretical part of the preparation is to provide detailed information on methodologies using the latest knowledge of the field with respect to the current state of routine application of new techniques and technologies. Fundamentals of indication and interpretation of laboratory methods in relation to clinical units and diagnoses. In the practical part of the training, gain practical skills of such a magnitude that allow the graduate to be easily assigned to clinical laboratory teams;
Learning outcomes
Upon completion of the course the student will be able to: actively assess the correctness of indication and interpretation of biochemical laboratory examination; to reliably detect and respond to instances of interference; communicate appropriately with clinicians; to respond appropriately to outlier findings; to have an overview of the used analytical principles and to know their limits; to follow standard analytical, technical and logistical operation guides.
Syllabus
  • Theoretical part of methodology
  • Non-laboratory and laboratory pre-analytical phase
  • Plasma proteins; albumin and specific proteins
  • Glucose and glycated hemoglobin
  • Cholesterol and its fractions, triacylglycerols, lipoproteins
  • linear enzymology; specific analytical features
  • Laboratory diagnosis of thyroid disorders; TSH, fT3,4, anti TPO
  • Laboratory diagnosis of disorders of the pituitary-adrenal axis
  • Reproductive hormones;
  • Parameters of acid-base and electrolyte metabolism; ISE, osmolality; acid-base analyzers
  • Parameters of oxygen metabolism;
  • Heart disease markers; ischemia, cardiac insufficiency
  • Methods of examination of renal function and nitrogen metabolism; glom. filtration, concentration capabilities, proteinuria
  • Methods of examination of liver function and disorders
  • Calcium, magnesium, phosphorus
  • Trace elements (Fe, Zn, Cu, Se ...) and vitamins (A, D, E, K ... C, B12,
  • Toxicology analyzes in clinical biochemistry
  • Therapeutic drug monitoring
  • Congenital disorders of metabolism; methods of neonatal screening
  • Methods for examination of urine, pleural effusion and ascites
  • Tumor markers; sensitivity, specificity, cut-off value
  • Cerebrospinal fluid examination methods; biochemical and cytomorphological diagnostics, intrathecal systhesis
  • Bone metabolism disorder markers
  • Principles of good laboratory practice and basics of quality management system; ISO 15189: 2013
  • Internal and external quality control; Westgard's rules
  • Post-analytical phase - disclosure of findings, data archiving
  • Laboratory information systems
  • Theoretical part of clinical education
  • Heart diseases
  • Diabetes mellitus; diagnosis, treatment, monitoring, insulin, C-peptide, o-GTT, microalbuminuria
  • Disorders of lipid metabolism; risk of atherosclerosis, target values
  • Bone metabolism, osteoporosis
  • Iron metabolic disorders, anemic conditions
  • Disorders of the internal environment, water, ions, osmolality, ABR-metabolic and respiratory disorders
  • Renal and urinary disorders, GF estimation, concentration,
  • Urolithiasis
  • Diseases of the digestive tract
  • Tumor diseases
  • Endocrine Diseases
  • Neurological diseases, inflammatory and degenerative diseases of the CNS
  • Reference limits and cut-off values ​​of laboratory methods
  • Principles of correct indication and interpretation of laboratory test methods
Literature
  • DASTYCH, Milan, Miroslava BEŇOVSKÁ, Petr BREINEK, Dana BUČKOVÁ, Zdeňka ČERMÁKOVÁ, Vladimír SOŠKA, Jana TŮMOVÁ, Jaroslava VÁVROVÁ, Miroslav VERNER and Hana VINOHRADSKÁ. Klinická biochemie - bakalářský obor Zdravotní laborant. 2. přepracované vydání. Brno: Masarykova Univerzita, 2011, 252 pp. ISBN 978-80-87192-18-4. info
  • Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood - David E. Bruns - Barbara G. Sawye. 6th ed. St. Louis: Saunders/Elsevier, 2008, xx, 952. ISBN 9780721638652. info
  • RACEK, Jaroslav. Klinická biochemie. 2., přeprac. vyd. Praha: Galén, 2006, 329 s. ISBN 8072623249. info
Teaching methods
lectures with audiovisual aids (.ppt, commented videos), interactive guidence
Assessment methods
two written tests, final oral examination with questions from clinical and analytical parts of the classes
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2015, Autumn 2016, Autumn 2017, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2018, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2018/MBKB071p