MBPA091p Pathology - lectures

Faculty of Medicine
autumn 2018
Extent and Intensity
1/0/0. 2 credit(s). Type of Completion: k (colloquium).
Teacher(s)
MUDr. Jitka Kyclová (lecturer)
PhDr. Květoslava Matulová (lecturer)
MUDr. Zdeňka Čermáková, Ph.D. (assistant)
Michaela Gregorovičová (assistant)
Guaranteed by
PhDr. Květoslava Matulová
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Timetable
Tue 12:00–13:40 ÚP P01014
Prerequisites
The student have to have completed basic required and selective courses comprising the advanced biochemical and biological subjects from 1st and 2nd semester.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is dedicated to the application of histological methodologies in practical patomorphological diagnostics (bioptic and necroptic). The student will get acquainted with the practical operation of the histopathological laboratory and will gain practical skills for the representation of individual tissue components by histological, histochemical and immunohistochemical methods as well as some molecular biological processes. The subject acquaints with modern methods of histopathological diagnostics of tumors and other patomorphological units.
Learning outcomes
Upon completion of the course the student will be skilled in recent histopathological techniques performed at the pathological workplaces, will be oriented in application of the appropriate laboratory procedures due to the nature and quality of the biological material and the diagnostic purpose.
Syllabus
  • Structure and work of the Institute of Pathology. Types and transport of biopsy material, biopsy handbook. Autopsy, structure and workload of autoptic pathology. Fixation, physical and chemical fixation, main types of fixation fluids. Coloring methods: hematoxylin-eosin, Azan, green trichrome, impregnation, dyeing Weigert van Gieson. Cytodiagnostics, types of cytological sampling, preparation of cytological specimens. Cytological diagnosis of the cervix, cytological screening. Histochemistry, basic types of reactions. Deep freezing, cryostat sections for histochemical reactions (enzymes, lipids, glycids). Preparation and slicing of tissue bottles on toboggan and rotary microtome, use of automatic and semi-automatic microtomes. Histochemical representation of enzymes, basic types of reactions. Detection of enzymes in malabsorption syndrome and myopathy. Lipid classification and histochemistry, extraction methods. Applications - steatosis, stinging disease. Glycid classification and histochemistry, application to gastrointestinal tract biopsy, glycogenesis. Histochemical representation of amyloid, Cu, and Fe. Principle and bases of immunohistochemical reactions, mono- and polyclonal antibodies. Intermediate filaments (cytokeratins, vimentin, desmin, GFAP, neurofilaments), significance for determination of basic types of tissues and their tumor derivatives. Steps of diagnostic procedure in immunohistochemical diagnostics of tumors. CD antigens and antibodies, applications in hematology and in the diagnosis of malignant lymphomas. Molecular pathology methods in diagnosis. Principle of fluorescence in situ hybridization (FISH), using this methodology. PCR Principle. Main advantages and disadvantages of this method. Differences (in the design and in the information they provide) between immunohistochemical analysis and immunoblotting.
Literature
    recommended literature
  • JIRKOVSKÁ, Marie. Histologická technika : pro studenty lékařství a zdravotnické techniky. 1. vyd. Praha: Galén, 2006, 80 s. ISBN 8072622633. info
    not specified
  • ČECH, Svatopluk and Drahomír HORKÝ. Histologie a mikroskopická anatomie pro bakaláře (Histology and microscopical anatomy for bachelors). 1. dotisk 2., přepr. vyd. Brno: Masarykova univerzita, 2017, 138 pp. ISBN 978-80-210-5544-5. info
  • BALKO, Jan, Zbyněk TONAR and Ivan VARGA. Memorix histologie. Edited by Radovan Hudák. 2. doplněné vydání. Praha: Triton, 2017, 555 stran. ISBN 9788075532497. info
  • HORKÝ, Drahomír and Svatopluk ČECH. Základy histologické techniky pro zdravotní laboranty (Basic histological technique for laboratory assistents). První. Brno: Vydavatelství Masyrykovy univerzity, 2007, 55 pp. ISBN 978-80-210-4467-8. info
  • Theory and practice of histological techniques. Edited by John D. Bancroft - Marilyn Gamble. 5th ed. Edinburgh: Churchill Livingstone, 2003, xiii, 796. ISBN 0443064350. info
Teaching methods
lecture with the use of audiovisual aids (.ppt, commented videos) including interactive inputs
Assessment methods
oral examination
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 15.
The course is also listed under the following terms Autumn 2015, Autumn 2016, Autumn 2017, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2018, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2018/MBPA091p