FAEP1_12 Explant Cultures

Faculty of Pharmacy
Autumn 2024

The course is not taught in Autumn 2024

Extent and Intensity
1/1/0. 3 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
Ing. Marcela Nejezchlebová (seminar tutor)
RNDr. Veronika Vaverková, Ph.D. (seminar tutor)
Guaranteed by
prof. PharmDr. Petr Babula, Ph.D.
Department of Natural Drugs – Departments – Faculty of Pharmacy
Prerequisites
FAKULTA(FaF) || OBOR(MUSFaF)
Fundamentals of Plant Physiology, Pharmaceutical Botany, Laboratory practice in the analytical laboratory.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 2 student(s).
Current registration and enrolment status: enrolled: 0/2, only registered: 0/2
fields of study / plans the course is directly associated with
Course objectives
Subject includes topics implying plant growth regulators and their physiological effects, explants cultures, basic methodes of fluorescent microscopy for work with explants cultures.
Learning outcomes
Understanding of basic processes, experiments, and exploitation of explant cultures.
Syllabus
  • Lectures:
    1. Introduction to plant cultures ? from back to future.
    2. Culture media and their composition, preparation and sterilization of explants.
    3. Types of explant cultures
    4. Initiation and maintenance of callus and suspension cultures
    5. Characterization of growth and development of callus and suspension cultures.
    6. Protoplast cultures ? isolation of protoplast, protoplast cultivation and characterization.
    7. Protoplast technologies ? fusion and selection.
    8. Selection of plant cells for desiderable characteristics ? mutant selection, cold tolerance, salt tolerance, disease/toxin resistance.
    9. Aspects of plant regeneration. Micropropagation.
    10. Cryopreservation and artificial seeds.
    11. Secondary metabolites from plant in vitro cultures I.
    12. Secondary metabolites from plant in vitro cultures II.
    13. Transgenic plants in pharmacy.

    Practical classes:
    1. Plant growwth regulators - biotests
    2. Preparation of cultivation media, establishing of plant cell tissue cultures
    3. Determination of growth and biosynthetical parameters of plant tissue cultures
    4. Methods of fluorescence microscopy in plant cell cultures
Literature
    required literature
  • Procházka, S., Šebánek, J. a kol. Regulátory rostlinného růstu. Academia praha, Praha, Česká republika, 1997. ISBN 8020005978. info
  • Dixon, R. A., Gonzales, R. A. Plant Cell Culture - A practical approach. Oxford university press, Oxford, Velká Británie, 1996. info
    not specified
  • Singh, R. J. Plant Cytogenetics. CRC Press, London, Velká Británie, 2003. ISBN 0849323886. info
  • Hawes, Ch., Satiat-Jeunemaitre, B. Plant Cell Biology. Oxford, Velká Británie, 2001. ISBN 0199638659. info
Language of instruction
English
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
Credit Requirements:
100% attendance at the exercise
  • Permalink: https://is.muni.cz/course/pharm/autumn2024/FAEP1_12