F1BL1_16 Biotechnology of Drugs

Faculty of Pharmacy
Spring 2022
Extent and Intensity
3/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
Mgr. Marie Brázdová, Ph.D. (lecturer)
doc. RNDr. Jan Hošek, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Jan Hošek, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Supplier department: Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable
Wed 10:20–12:50 44-056
Prerequisites
The knowledge of subject in range of lectures
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 80 student(s).
Current registration and enrolment status: enrolled: 3/80, only registered: 0/80, only registered with preference (fields directly associated with the programme): 0/80
fields of study / plans the course is directly associated with
Course objectives
This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.
Learning outcomes
Basic knowledge from cytology, genetic engineering, and industrial microbiology which are used in preparation of recombinant pharmaceuticals. Overview of biotechnology production of antibiotics, vitamins, organic acids, amino acids and alkaloids.
Syllabus
  • 1) Introduction to pharmaceutical biotechnology, definition, milestones
    2) The cell as a tool of biotechnology, function of endoplasmic reticulum, Golgi komplex, posttranslational processes, chaperones
    3) The basics of gene engineering I - the principles, how to prepare recombinant gene, vectors
    4) The basics of gene engineering II - preparation of genes for cloning, host cells, transformation, selection and transformants identification
    5) Expression of recombinant proteins in prokaryotic cells and yeasts
    6) Expression of recombinant proteins in eukaryotic cells - insect cells and baculoviruses, mammalian cells and adenoviruses
    7) The classical biotechnological processes in pharmacology
    8) The process of biotechnology - definition, the phases of biotechnological process, basic materials, fermentors and bioreactors, methods of biotechnological products purification
    9) Examples of classical and recombinant biotechnology products in pharmacy, cytokines, hormones, enzymes, antibodies and their derivates
    10) Gene engineering of plants - structure of plant genome, vectors, expression cassettes
    11) Plant biotechnology - methods of transformation and transgene identification, molecular pharming
    12) Application of gene engineering and plant biotechnology in pharmacy, medicine, and food industry, rules for genetically modified organisms manipulation
    13) Gene and cell therapy, tissue engineering, genomics and proteomics in pharmacy
    14) New trends in biopharmaceutical products development
Literature
    required literature
  • Brawn. Klonování genů a analýza DNA. Univerzita Palackého v Olomouci,, 2007. info
  • Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info
  • Ruml, T.et al. Genové inženýrství. VŠCHT Praha, 2002. info
  • Ondřej, M., Drobník, J. Transgenoze rostlin. Academia Praha, 2002. info
  • Vodrážka, Z. Biotechnologie. VŠCHT Praha, 1991. info
    recommended literature
  • Alberts et al. Základy buněčné biologie, Úvod do molekulární biologie buňky. Espero Publishing, 1998. info
    not specified
  • KAYSER, O., MÜLLER, R.H. Pharmaceutical Biotechnology. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim, Ge, 2004. info
  • HO, R. J. Y. , GIBALDI, M. Biotechnology and Biopharmaceuticals. Transforming proteins and genes into drugs. John Willey and Sons, Hobokey, New Jersey, U.S.A, 2003. info
  • SLATER, A., SCOTT, N., FOWLER, M. Plant biotechnology. The genetic manipulation of plants. Oxford University Press. Oxford, New York, U.S.A, 2003. info
Teaching methods
lectures, class discussion,
Assessment methods
Written and oral exam.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2020, Spring 2021, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/pharm/spring2022/F1BL1_16