FABL1_16 Biotechnology of Drugs

Farmaceutická fakulta
jaro 2024
Rozsah
3/0/0. 4 kr. Ukončení: zk.
Vyučující
Mgr. Marie Brázdová, Ph.D. (přednášející)
doc. RNDr. Jan Hošek, Ph.D. (přednášející)
Garance
doc. RNDr. Jan Hošek, Ph.D.
Ústav molekulární farmacie – Ústavy – Farmaceutická fakulta
Rozvrh
Po 9:00–11:30 45-224
Předpoklady
The knowledge of subject in range of lectures.
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.

Předmět si smí zapsat nejvýše 20 stud.
Momentální stav registrace a zápisu: zapsáno: 4/20, pouze zareg.: 0/20
Mateřské obory/plány
Cíle předmětu
The course is demonstration of Pharmaceutical Biotechnology - scientific discipline focusing on all technologies required for the production, manufacturing and registration of biotechnological drugs. Students will be introduced to processes which are used by organisms or their parts to production or modification of drugs. The methods used for breeding of animals, plants and microorganisms usable in medicine and pharmacy will be discussed. The main focus is done for recombinant techniques, gene and protein engineering and gene therapy. Products of industrial biotechnology as antibiotics, vitamins, organic acids, amino acids and alkaloids, will be presented.
Výstupy z učení
Basic knowledge from cytology, gene engineering and industrial microbiology usable in recombinant drugs design, development and production. View to biotechnological production of antibiotics, vitamins, organic acids, amino acids and alkaloids.
Osnova
  • 1) Introduction to pharmaceutical biotechnology, definition, milestones
    2) The cell as a tool of biotechnology, function of endoplasmic reticulum, Golgi komplex, posttranslational processes, chaperones
    3) The basics of gene engineering I - the principles, how to prepare recombinant gene, vectors
    4) The basics of gene engineering II - preparation of genes for cloning, host cells, transformation, selection and transformants identification
    5) Expression of recombinant proteins in prokaryotic cells and yeasts
    6) Expression of recombinant proteins in eukaryotic cells - insect cells and baculoviruses, mammalian cells and adenoviruses
    7) The classical biotechnological processes in pharmacology
    8) The process of biotechnology - definition, the phases of biotechnological process, basic materials, fermentors and bioreactors, methods of biotechnological products purification
    9) Examples of classical and recombinant biotechnology products in pharmacy, cytokines, hormones, enzymes, antibodies and their derivates
    10) Gene engineering of plants - structure of plant genome, vectors, expression cassettes
    11) Plant biotechnology - methods of transformation and transgene identification, molecular pharming
    12) Application of gene engineering and plant biotechnology in pharmacy, medicine, and food industry, rules for genetically modified organisms manipulation
    13) Gene and cell therapy, tissue engineering, genomics and proteomics in pharmacy
    14) New trends in biopharmaceutical products development
Literatura
    povinná literatura
  • Brown, T.A. (2010). Gene cloning and DNA analysis, an introduction. 6th edition, Wiley-Blackwell. info
  • Alberts et al. (1998). Molecular Biology of the Cell, 5th edition, GS Garland Science. info
  • Walsh, G. (2007). Pharmaceutical Biotechnology, John Wiley and sons. info
  • HO, R. J. Y. , GIBALDI, M. Biotechnology and Biopharmaceuticals. Transforming proteins and genes into drugs. John Willey and Sons, Hobokey, New Jersey, U.S.A, 2003. info
  • SLATER, A., SCOTT, N., FOWLER, M. Plant biotechnology. The genetic manipulation of plants. Oxford University Press. Oxford, New York, U.S.A, 2003. info
    doporučená literatura
  • Alberts et al. Essential cell biology. Garland Science, Taylor and Francis Group. ISBN 0-8153-3481-8. info
    neurčeno
  • KAYSER, O., MÜLLER, R.H. Pharmaceutical Biotechnology. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim, Ge, 2004. info
Výukové metody
lectures, videos
Metody hodnocení
Written examination test and oral examination
Vyučovací jazyk
Angličtina
Další komentáře
Studijní materiály
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Nachází se v prerekvizitách jiných předmětů
Předmět je zařazen také v obdobích jaro 2020, jaro 2021, jaro 2025.
  • Statistika zápisu (nejnovější)
  • Permalink: https://is.muni.cz/predmet/pharm/jaro2024/FABL1_16