F1MG1_16 Methods of Molecular Biology

Faculty of Pharmacy
Autumn 2023
Extent and Intensity
1/0/0. 2 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Jan Hošek, Ph.D. (lecturer)
Mgr. Marie Brázdová, Ph.D. (lecturer)
Mgr. Daniel Renčiuk, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Jan Hošek, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Supplier department: Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable
each odd Thursday 9:55–11:35 45-135
Prerequisites
FAKULTA(FaF) || OBOR(MUSFaF)
Molecular biology.The knowledge of subject in range of lectures.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 100 student(s).
Current registration and enrolment status: enrolled: 79/100, only registered: 0/100, only registered with preference (fields directly associated with the programme): 0/100
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to interpret the basic principles of molecular biology methods and to practice the basic operations in the molecular biology used.
Learning outcomes
The aim of this subject is to provide students with basic information from molecular biology methods. The ground of lectures is focused on structure of proteins and nucleic acids and functions and relationships of these informational macromolecules in transition of genetic information.
Syllabus
  • 1) Isolation and characterisation of nucleic acids and proteins
    2) Centrifugation
    3) Electrophoresis - agarose, polyacrylamide, pulse-field, capillary
    4) Modifying enzymes
    5) Restriction endonucleases
    6) Polymerase chain reaction including real-time PCR, Real-time PCR
    7) Southern a northern blotting, hybridisation, DNA and RNA chips
    8) Sequencing of DNA and proteins
    9) ELISA, western blotting proteomics
    10) Transfection

    11) Analysis of protein-protein interactions,
    12) Analysis of DNA-protein interaction 13) Reporter systems
Literature
    required literature
  • Šmarda J, et al. Metody molekulární biologie. Masarykova Universita Brno, 2005. info
    recommended literature
  • Barker K. At the Bench. A laboratory navigator. Cold Spring Harbor Laboratory Press, USA, 2005. info
    not specified
  • Bustin SA. A-Z of quantitative PCR. International University Line, USA, 2004. info
  • Sambrook a Russell. Molecular cloning, A laboratory manual. the 3rd edition. Cold Spring Harbor Laboratory Press, New York, U, 2001. info
Teaching methods
lectures, videos
Assessment methods
Written exam, to successfully complete the need to master 60%.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is also listed under the following terms Autumn 2020, Autumn 2021, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/pharm/autumn2023/F1MG1_16