Bi7039 Work in BSL3 and BSL4 Laboratories

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 4 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
prof. RNDr. Daniel Růžek, Ph.D. (lecturer)
doc. RNDr. Luděk Eyer, Ph.D. (lecturer)
RNDr. Jiří Salát, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Daniel Růžek, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
The subject is designated for virology students.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goal of this course is to gain theoretical and practical information about the work in laboratories with higher (BSL3) and the highest (BSL4) level of biological protection. Students will be familiar with the construction and function of BSL3 and BSL4 laboratories, will know the rules for work, personal protective equipment, how to handle and accident. Students will visit functional BSL3 and BSL4 laboratories.
Learning outcomes
Students will know theoretically and practically the specifics of BSL3 and BSL4 laboratories, will be able to work in these laboratories, will know how to use the personal protective equipment and will know how to handle an acceident in these laboratores.
Syllabus
  • 1. Construction of the labs. Equipment.
  • 2. Safety work with infectious materiál.
  • 3. BSL3 and BSL4 – history and overview.
  • 4. Types of biological cabinets.
  • 5. Personal protective equipment.
  • 6. Four primary controls of biological safety.
  • 7. Decontamination.
  • 8. Accidents.
  • 9. Key tips for working in a virological laboratory.
  • 10. SOPs.
  • 11. Practical course.
  • 12. Visir of BSL3 and BSL4 laboratory.
Literature
    recommended literature
  • WHO. WHO: Laboratory biosafety manual. 4th ed. 2020. ISBN 978-92-4-001131-1. info
Teaching methods
The practical course takes place in the form of block teaching and combines theoretical lectures and practical demonstrations and excusion to BSL3 and BSL4 laboratory.
Assessment methods
The participation in the practical course is mandatory. At the end, there will be a final test.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught in blocks.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2025/Bi7039