Bi7330 Cytology and morphology of bacteria

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
In-person direct teaching
Teacher(s)
doc. Ivan Kushkevych, Ph.D. (lecturer)
Guaranteed by
doc. Ivan Kushkevych, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 17. 2. to Sat 24. 5. Mon 12:00–13:50 E25/209
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course, students will understand the problematics of cytology and morphology of bacterial cells and the techniques of microscopic and macroscopic assessments of bacterias. The aim of the course is also to involve students in the discussion of the problem.
Learning outcomes
Students will be able to characterize cytological structure, their functions and stages in more or less complex life-cycles. Student will be also able to consider the formation of the bacterial biofilm and its importance and signal processes and interactions between bacterial cells. For a specific purpose, students will be able to consider the suitability and applicability of particular methods - microscopy, archiving of acquired material, processing and analysis of images. Abilities from that field will be useful not only in biological sciences, but will also help to understand the physiological processes of bacterial cells.
Syllabus
  • Subject and tasks of the special course “Cytology and morphology of bacteria” and its place in the system of biological disciplines. Characteristics of research objects.
  • Methods of studying cell structure. Microscopic technique, light, fluorescence and electron microscopy. Phase and Nomarsky contrast.
  • Chemical composition of a microbial cell. Transport of substances into bacterial cells.
  • Differences between a prokaryotic and eukaryotic cell. General characteristics of the cell structure of prokaryotes.
  • Structure and chemical structure of the cell wall of eubacteria. Acid resistance of bacteria.
  • Characteristics of prokaryotic cells without a cell wall. Protoplasts and spheroplasts.
  • Surface structures of a bacterial cell. Capsules, sheaths and mucus in bacteria. Organelles of movement. Taxa.
  • Cytoplasmic membrane. Intracellular membrane structures of prokaryotes. Ribosomes, inclusions.
  • Genetic apparatus of bacteria: nucleoid, plasmids, mobile genetic elements.
  • Growth and reproduction of prokaryotic cells. Differentiation of bacterial cells. Spore formation in bacteria.
  • Bacterial cell cycles.
  • Dimorphic protozoan bacteria. Chlamydia.
  • Actinomycetes, myxobacteria.
  • Cyanobacteria
  • The importance of the molecular structure of individual parts of the bacterial cell in the classification and identification of bacteria.
Literature
  • PERRY, Jerome J. and James T. STALEY. Microbiology : dynamics and diversity. Fort Worth: Saunders College Publishing, 1997, xxxi, 911. ISBN 0030538939. info
Teaching methods
theoretical preparing for laboratory work in practical course of Cytology and Morphology of bacteria using illustrative schemes and image documentation
Assessment methods
recommended lectures, training in writing abstracts, oral colloquium
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
Teacher's information
http://www.sci.muni.cz/mikrob/cytologieosn.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Spring 2020, Spring 2021, Spring 2022.
  • Enrolment Statistics (recent)
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