Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Milan Bartoš, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 17. 9. to Fri 14. 12. Thu 14:00–15:50 E25/339
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
autumn 2017
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Milan Bartoš, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Thu 9:00–10:50 E25/339
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2016
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Milan Bartoš, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 19. 9. to Sun 18. 12. Thu 8:00–9:50 E25/339
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2015
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Mgr. Jana Čmielová, Ph.D. (lecturer)
Mgr. Eva Šebestová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Milan Bartoš, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 15:00–16:50 E25/339
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2014
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Mgr. Jana Čmielová, Ph.D. (lecturer)
Mgr. Eva Šebestová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Milan Bartoš, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 8:00–9:50 E25/339
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2012
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Mgr. Eva Šebestová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 8:00–9:50 Bpt,01013
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
Teaching methods
The teaching methods make use of presentations on stencils and in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2011
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Tue 8:00–9:50 Bpt,01013
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
Teaching methods
The teaching methods make use of presentations on stencils and in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2010
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Tue 10:00–11:50 Bpt,01013
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
Teaching methods
The teaching methods make use of presentations on stencils and in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Wed 8:00–9:50 Bpt,01013
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
Teaching methods
The teaching methods make use of presentations on stencils and in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Tue 8:00–9:50 Bpt,01013
Prerequisites
The course is for the students with interest in advanced molecular genetic and diagnostic methods used in analysis of microorganisms. Prerequisits are exams from Melecular Biology (Bi4020) and General mikrobiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Methods of the study of microbial cells - cultivation and noncultivation methods. DNA probes (characterisation and use,.labelling and detection). Experimental arrangements during hybridisations (hybridisations in solution and on solid phases, fluorescent in situ hybridisation). Microchips, their construction and use in microbiology. Amplification methods in molecular diagnostics of microorganisms (amplification of target DNA sequence, probe, signal carried on a probe). Real time PCR and detection of PCR products, (SybrGreen, TaqMan probes, hybridisation probes, molecular beacons). Enzymes used for manipulation with nucleic acids and thier use in construction of restriction maps. Molecular typisation methods in mikrobiology based on DNA and protein analysis. Methods of sequencing of nucleic acids and analysis of DNA sequences. Metagenomics - analysis of microbial communities. Molecular diagniostics of microorganisms with use of magnetic carriers. Search for biologically active compounds using of microorganisms. Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
At the end of this course the student will be able to understand the use of advanced molekular genetic methods in mikrobiology, to find suitable method for solving of conrete problem and to interpret results of analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
Assessment methods
Lectures are enclosed by oral exam.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2007
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Tue 8:00–9:50 Bpt,01013
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Preparation of recombinat DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms.Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistance to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity and the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Syllabus
  • Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms. Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistence to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity an d the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Literature
  • Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.
Assessment methods (in Czech)
Přednáška je ukončena zkouškou.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Mon 11:00–12:50 Bpt,01013
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Preparation of recombinat DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms.Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistance to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity and the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Syllabus
  • Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms. Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistence to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity an d the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Literature
  • Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.
Assessment methods (in Czech)
Přednáška je ukončena zkouškou.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2005
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Preparation of recombinat DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms.Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistance to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity and the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Syllabus
  • Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms. Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistence to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity an d the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Literature
  • Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.
Assessment methods (in Czech)
Přednáška je ukončena zkouškou.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Timetable
Thu 12:00–13:50 Bpt,01013
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Preparation of recombinat DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms.Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistance to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity and the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Syllabus
  • Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms. Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistence to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity an d the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Literature
  • Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.
Assessment methods (in Czech)
Přednáška je ukončena zkouškou.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Preparation of recombinat DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms.Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistance to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity and the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Syllabus
  • Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms. Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistence to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity an d the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Literature
  • Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.
Assessment methods (in Czech)
Přednáška je ukončena zkouškou.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Prerequisites (in Czech)
Bi6721 Spec. Meth. Microorg. Anal. I.
Předpokladem zapsání je zkouška z předmětu Speciální metody analýzy mikroorganismů I (B6721).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
Course objectives
Preparation of recombinat DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms.Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistance to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity and the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Syllabus
  • Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms. Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistence to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity an d the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Literature
  • Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.
Assessment methods (in Czech)
Přednáška je ukončena zkouškou, cvičení je ukončeno zápočtem.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Special Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2019

The course is not taught in Autumn 2019

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Pavel Dvořák, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Pavel Dvořák, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6721 Spec. Meth. Microorg. Anal. I.
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Learning outcomes
Student is able to apply 1)Real-time PCR methods in microbiology 2)Next generation sequencing and Third generation sequencing 3)Recombinant molecules in analysis of microbial genomes 4)Metagenomics in microbial communities’ research 5)Requirements of Good laboratory practise in research, development and applied laboratory
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
General note: Předmět je možné zapsat pouze ve 3. semestru.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2013

The course is not taught in Autumn 2013

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Milan Bartoš, Ph.D. (lecturer)
Mgr. Eva Šebestová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Wed 14:00–15:50 Bpt,01013
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
    recommended literature
  • Molecular microbiology : diagnostic principles and practice. Edited by David H. Persing. 2nd ed. Washington, DC: ASM Press, 2011, xvi, 936. ISBN 9781555814977. info
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
Teaching methods
The teaching methods make use of presentations in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
Teaching methods
The teaching methods make use of presentations on stencils and in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Prerequisites
The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. The prerequisits are exams from the courses Molecular Biology (Bi4020) and General Microbiology (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to compare and explain principles of advanced molecular genetic methods; to select and use methods suitable for the solution of a specific problem od microorganisms analysis; to interpret and evaluate the results of the analysis.
Syllabus
  • Technology of recombinant DNA and their importance for study of microorganisms.
  • Preparation of recombinat DNA molecules for diagnostic purposes. Nucleic acid probes, their characterisation and use.
  • Labelling and detection of probes.
  • Different arrangements during hybridisation. Hybridisation in solution and on solid phase. Fluorescent in situ hybridisation.
  • Microarrays (microchips), their construction and use in microbiology.
  • Amplification methods in molecular diagnostics of microorganisms. Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe.
  • PCR in real time. Detection of amplicons in real time PCR. TaqMan probes, hybridisation probes, molecular beacons.
  • Enzymes used in manipulation with nucleic acids. Restriction maps. Detection of polymorphisms in genomes.
  • Molecular typisation methods in mikrobiology. DNA fingerprints. Protein analysis and protein fingerprints.
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences.
  • Analysis of microbial communities – metagenomics. Use of magnetic microspheres in molecular diagniostics of microorganisms.
  • Use of microorganisms in search for biologically aktive compounds.
  • Validation of laboratory methods in microbiological laboratory. Acreditation of laboratory.
Literature
Teaching methods
The teaching methods make use of presentations on stencils and in PowerPoint. Presentations of the results of the student’s own analysis of real samples are included and used for the explanation of problems.
Assessment methods
The course is closed with a spoken exam. During the spoken exam the student is posed 3 questions. The overall result of the exam is evaluated by 2 examiners.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.

Bi7722 Methods of Microorganisms Analysis II

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Alena Španová, CSc. (lecturer)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
RNDr. Marie Vojtíšková, CSc. (lecturer)
Guaranteed by
doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Preparation of recombinat DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms.Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistance to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity and the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Syllabus
  • Preparation of recombinant DNA molecules for diagnostic purposes. Use of artificial gene transfer in the preparation of DNA probes. Electrotransformation. Molecular diagnostics of microorganisms. Amplification techniques in molecular diagnostics. Methods of molecular typisation. Identification of bacterial genes of resistence to antibiotics. Identification of genetically modified organisms (GMO) in foods. Methods enabling the determination of DNA fragment specificity an d the detection of mutations. Bioinformatics. Use of the analysis of bacterial DNA sequences. Validation of microbiological laboratory methods.
Literature
  • Woodford N., Johnson A.P. (1998) Molecular Bacteriology: Protocols and clinical applications. Humana Press, Totowa, New Yersey. 2. Pressing D.H. (Ed.) (1993) Diagnostic Molecular Microbiology: Principles and Applications. ASM Press, Washington.
Assessment methods (in Czech)
Přednáška je ukončena zkouškou.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (recent)