Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2025
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
Mgr. Ing. Eliška Kučerová (assistant)
Guaranteed by
Mgr. Tibor Botka, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Tibor Botka, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 19/15
fields of study / plans the course is directly associated with
Course objectives
The course includes preparation and cultivation techniques of bacterial viruses: Preparation of phage lysate; Determination of the virion titer; One-step growth curve and phage burst size determination by quantitative PCR; Determination of mutation index; Induction of prophages using mitomycin C; Isolation of spontaneous kayvirus mutants on resistant Staphylococcus aureus strains; comparative analysis of changes in viral genomes by PCR, sequencing, and bioinformatics; Purification and concentration of viral particles. After graduation this course, students should be able to conduct and fully understand experiments, from the acquisition of input material to the analysis and interpretation of experimental data.
Learning outcomes
At the end of the course students should be able to:
• isolate viral particles and DNA in sufficient quality and quantity
• analyze isolated genetic material by methods of molecular biology such as PCR, qPCR, ELFO, DNA sequencing, and bioinformatics tools
• isolate and prepare lysate of spontaneous viral mutants effective on up-to-now phage-resistant bacterial strain
• determine and compare the growth properties of bacterial viruses
• determine and compare the growth properties of lysogenic strains depending on the type of prophage and the mode of its induction used
• use theoretical knowledge of other methods and principles in subsequent laboratory practice
Syllabus
  • 1. Isolation of spontaneous mutants of bacterial viruses, determination of mutation frequency and sequence analysis 2. Determination of susceptibility of Staphylococcus aureus strains to polyvalent Kayvirus mutants 3. One-step growth curve and adsorption curve of staphylococcal virus 812 and its mutants, determination of burst size by qPCR 4. Observation of growth properties of lysogenic bacteria using spectrophotometer, induction using mitomycin C and isolation of resident bacterial viruses 5. Purification of viral particles by various ultrafiltration techniques
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is successfully completed if the student completes all exercises, passes a written test and submits all protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Teacher's information
Students would independently manage the entered tasks and corectly interpret the results in laboratory protocols.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2024
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
Mgr. Ing. Eliška Kučerová (assistant)
Guaranteed by
Mgr. Tibor Botka, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Tibor Botka, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8312/01: Mon 19. 2. to Sun 26. 5. Tue 9:00–12:50 E25/119, T. Botka
Bi8312/02: Mon 19. 2. to Sun 26. 5. Tue 9:00–12:50 E25/119, T. Botka
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 6/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
The course includes preparation and cultivation techniques of bacterial viruses: Preparation of phage lysate; Determination of the virion titer; One-step growth curve and phage burst size determination by quantitative PCR; Determination of mutation index; Induction of prophages using mitomycin C; Isolation of spontaneous kayvirus mutants on resistant Staphylococcus aureus strains; comparative analysis of changes in viral genomes by PCR, sequencing, and bioinformatics; Purification and concentration of viral particles. After graduation this course, students should be able to conduct and fully understand experiments, from the acquisition of input material to the analysis and interpretation of experimental data.
Learning outcomes
At the end of the course students should be able to:
• isolate viral particles and DNA in sufficient quality and quantity
• analyze isolated genetic material by methods of molecular biology such as PCR, qPCR, ELFO, DNA sequencing, and bioinformatics tools
• isolate and prepare lysate of spontaneous viral mutants effective on up-to-now phage-resistant bacterial strain
• determine and compare the growth properties of bacterial viruses
• determine and compare the growth properties of lysogenic strains depending on the type of prophage and the mode of its induction used
• use theoretical knowledge of other methods and principles in subsequent laboratory practice
Syllabus
  • 1. Isolation of spontaneous mutants of bacterial viruses, determination of mutation frequency and sequence analysis 2. Determination of susceptibility of Staphylococcus aureus strains to polyvalent Kayvirus mutants 3. One-step growth curve and adsorption curve of staphylococcal virus 812 and its mutants, determination of burst size by qPCR 4. Observation of growth properties of lysogenic bacteria using spectrophotometer, induction using mitomycin C and isolation of resident bacterial viruses 5. Purification of viral particles by various ultrafiltration techniques
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is successfully completed if the student completes all exercises, passes a written test and submits all protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
Students would independently manage the entered tasks and corectly interpret the results in laboratory protocols.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2023
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Tibor Botka, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Tibor Botka, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8312/01: Tue 8:00–11:50 E25/119, T. Botka
Bi8312/02: Tue 8:00–11:50 E25/119, T. Botka
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 5/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
The course includes preparation and cultivation techniques of bacterial viruses: Preparation of phage lysate; Determination of the virion titer; One-step growth curve and phage burst size determination by quantitative PCR; Determination of mutation index; Induction of prophages using mitomycin C; Isolation of spontaneous kayvirus mutants on resistant Staphylococcus aureus strains; comparative analysis of changes in viral genomes by PCR, sequencing, and bioinformatics; Purification and concentration of viral particles. After graduation this course, students should be able to conduct and fully understand experiments, from the acquisition of input material to the analysis and interpretation of experimental data.
Learning outcomes
At the end of the course students should be able to:
• isolate viral particles and DNA in sufficient quality and quantity
• analyze isolated genetic material by methods of molecular biology such as PCR, qPCR, ELFO, DNA sequencing, and bioinformatics tools
• isolate and prepare lysate of spontaneous viral mutants effective on up-to-now phage-resistant bacterial strain
• determine and compare the growth properties of bacterial viruses
• determine and compare the growth properties of lysogenic strains depending on the type of prophage and the mode of its induction used
• use theoretical knowledge of other methods and principles in subsequent laboratory practice
Syllabus
  • 1. Isolation of spontaneous mutants of bacterial viruses, determination of mutation frequency and sequence analysis 2. Determination of susceptibility of Staphylococcus aureus strains to polyvalent Kayvirus mutants 3. One-step growth curve and adsorption curve of staphylococcal virus 812 and its mutants, determination of burst size by qPCR 4. Observation of growth properties of lysogenic bacteria using spectrophotometer, induction using mitomycin C and isolation of resident bacterial viruses 5. Purification of viral particles by various ultrafiltration techniques
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is successfully completed if the student completes all exercises, passes a written test and submits all protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
Students would independently manage the entered tasks and corectly interpret the results in laboratory protocols.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2022
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8312/01: Wed 8:00–11:50 E25/119, T. Botka
Bi8312/02: Wed 8:00–11:50 E25/119, T. Botka
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
The course includes preparation and cultivation techniques of bacterial viruses: Preparation of phage lysate; Determination of the virion titer; One-step growth curve and phage burst size determination by quantitative PCR; Determination of mutation index; Induction of prophages using mitomycin C; Isolation of spontaneous kayvirus mutants on resistant Staphylococcus aureus strains; comparative analysis of changes in viral genomes by PCR, sequencing, and bioinformatics; Purification and concentration of viral particles. After graduation this course, students should be able to conduct and fully understand experiments, from the acquisition of input material to the analysis and interpretation of experimental data.
Learning outcomes
At the end of the course students should be able to:
• isolate viral particles and DNA in sufficient quality and quantity
• analyze isolated genetic material by methods of molecular biology such as PCR, qPCR, ELFO, DNA sequencing, and bioinformatics tools
• isolate and prepare lysate of spontaneous viral mutants effective on up-to-now phage-resistant bacterial strain
• determine and compare the growth properties of bacterial viruses
• determine and compare the growth properties of lysogenic strains depending on the type of prophage and the mode of its induction used
• use theoretical knowledge of other methods and principles in subsequent laboratory practice
Syllabus
  • 1. Isolation of spontaneous mutants of bacterial viruses, determination of mutation frequency and sequence analysis 2. Determination of susceptibility of Staphylococcus aureus strains to polyvalent Kayvirus mutants 3. One-step growth curve and adsorption curve of staphylococcal virus 812 and its mutants, determination of burst size by qPCR 4. Observation of growth properties of lysogenic bacteria using spectrophotometer, induction using mitomycin C and isolation of resident bacterial viruses 5. Purification of viral particles by various ultrafiltration techniques
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is successfully completed if the student completes all exercises, passes a written test and submits all protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
Teacher's information
Students would independently manage the entered tasks and corectly interpret the results in laboratory protocols.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2021
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi8312/B1: Mon 14. 6. 8:00–12:00 E25/119, Tue 15. 6. 8:00–12:00 E25/119, Wed 16. 6. 8:00–12:00 E25/119, Thu 17. 6. 8:00–12:00 E25/119
Bi8312/B2: Mon 21. 6. 8:00–12:00 E25/119, Tue 22. 6. 8:00–12:00 E25/119, Wed 23. 6. 8:00–12:00 E25/119, Thu 24. 6. 8:00–12:00 E25/119
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 1/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
The course includes preparation and cultivation techniques of bacterial viruses: Preparation of phage lysate; Determination of the virion titer; One-step growth curve and phage burst size determination by quantitative PCR; Determination of mutation index; Induction of prophages using mitomycin C; Isolation of spontaneous kayvirus mutants on resistant Staphylococcus aureus strains; comparative analysis of changes in viral genomes by PCR, sequencing, and bioinformatics; Purification and concentration of viral particles. After graduation this course, students should be able to conduct and fully understand experiments, from the acquisition of input material to the analysis and interpretation of experimental data.
Learning outcomes
At the end of the course students should be able to:
• isolate viral particles and DNA in sufficient quality and quantity
• analyze isolated genetic material by methods of molecular biology such as PCR, qPCR, ELFO, DNA sequencing, and bioinformatics tools
• isolate and prepare lysate of spontaneous viral mutants effective on up-to-now phage-resistant bacterial strain
• determine and compare the growth properties of bacterial viruses
• determine and compare the growth properties of lysogenic strains depending on the type of prophage and the mode of its induction used
• use theoretical knowledge of other methods and principles in subsequent laboratory practice
Syllabus
  • 1. Isolation of spontaneous mutants of bacterial viruses, determination of mutation frequency and sequence analysis 2. Determination of susceptibility of Staphylococcus aureus strains to polyvalent Kayvirus mutants 3. One-step growth curve and adsorption curve of staphylococcal virus 812 and its mutants, determination of burst size by qPCR 4. Observation of growth properties of lysogenic bacteria using spectrophotometer, induction using mitomycin C and isolation of resident bacterial viruses 5. Purification of viral particles by various ultrafiltration techniques
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is successfully completed if the student completes all exercises, passes a written test and submits all protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
Teacher's information
Students would independently manage the entered tasks and corectly interpret the results in laboratory protocols.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2020
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
The course includes preparation and cultivation techniques of bacterial viruses: Preparation of phage lysate; Determination of the virion titer; One-step growth curve and phage burst size determination by quantitative PCR; Determination of mutation index; Induction of prophages using mitomycin C; Isolation of spontaneous kayvirus mutants on resistant Staphylococcus aureus strains; comparative analysis of changes in viral genomes by PCR, sequencing, and bioinformatics; Purification and concentration of viral particles. After graduation this course, students should be able to conduct and fully understand experiments, from the acquisition of input material to the analysis and interpretation of experimental data.
Learning outcomes
At the end of the course students should be able to:
• isolate viral particles and DNA in sufficient quality and quantity
• analyze isolated genetic material by methods of molecular biology such as PCR, qPCR, ELFO, DNA sequencing, and bioinformatics tools
• isolate and prepare lysate of spontaneous viral mutants effective on up-to-now phage-resistant bacterial strain
• determine and compare the growth properties of bacterial viruses
• determine and compare the growth properties of lysogenic strains depending on the type of prophage and the mode of its induction used
• use theoretical knowledge of other methods and principles in subsequent laboratory practice
Syllabus
  • 1. Isolation of spontaneous mutants of bacterial viruses, determination of mutation frequency and sequence analysis 2. Determination of susceptibility of Staphylococcus aureus strains to polyvalent Kayvirus mutants 3. One-step growth curve and adsorption curve of staphylococcal virus 812 and its mutants, determination of burst size by qPCR 4. Observation of growth properties of lysogenic bacteria using spectrophotometer, induction using mitomycin C and isolation of resident bacterial viruses 5. Purification of viral particles by various ultrafiltration techniques
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is successfully completed if the student completes all exercises, passes a written test and submits all protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
Teacher's information
Students would independently manage the entered tasks and corectly interpret the results in laboratory protocols.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2019
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of its mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construction of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poisson's distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
spring 2018
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of its mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construction of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poisson's distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2017
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of its mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construction of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poisson's distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2016
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of its mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construction of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poisson's distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2015
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of its mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construction of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poisson's distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2014
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of its mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construction of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poisson's distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as a practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the IS.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2013
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi6400 Methods of molecular biology
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as the practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the Is.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2012
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as the practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the Is.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2011
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Mgr. et Mgr. Veronika Oškerová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as the practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the Is.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2010
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as the practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the Is.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2009
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently work with the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods
Type of the practical exercise: Type of credit: execution, carrying out the methods, written protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2008
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2007
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2006
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && Bi7311 Mol. biol. of procar.-practice && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses )
1. Basic methods in molecular biology 2. Molecular biology of DNA and RNA viruses 3. Life and reproduction of bacteriophages
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2005
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && Bi7311 Mol. biol. of procar.-practice && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses )
1. Basic methods in molecular biology 2. Molecular biology of DNA and RNA viruses 3. Life and reproduction of bacteriophages
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2004
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && Bi7311 Mol. biol. of procar.-practice && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses )
1. Basic methods in molecular biology 2. Molecular biology of DNA and RNA viruses 3. Life and reproduction of bacteriophages
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2003
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && Bi7311 Mol. biol. of procar.-practice && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses )
1. Basic methods in molecular biology 2. Molecular biology of DNA and RNA viruses 3. Life and reproduction of bacteriophages
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as the practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the Is.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages. After graduation this course, the student should be able to competently work with the viruses originating from the bacteria. After graduation this course, the student should be able to competently analyse the bacteriophages and their genetic material.
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Teaching methods
This course runs as the practical exercise. Students conduct the experiments in assignments ranked according to course syllabus. Study materials are available in the Is.
Assessment methods
The course is completed and creditted after work out the laboratory minutes and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

Bi8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( B7140 Molecular biology of viruses || Bi7140 Molecular biology of viruses ) && ! Bi5491 Molecular Diagnostics
1. Basic methods in molecular biology; 2. Molecular biology of DNA and RNA viruses; 3. Life and reproduction of bacteriophages.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
fields of study / plans the course is directly associated with
Course objectives
Course contains preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Prosíme striktně o dodržování limitu počtu studentů
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (recent)