Bi8360c Molecular diagnostics of microorganisms - practice

Faculty of Science
Spring 2025

The course is not taught in Spring 2025

Extent and Intensity
0/2. 2 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites (in Czech)
Bi6405 Molecular Methods - practice && NOW( Bi8360 Molecular diagnostics of micro )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Syllabus (in Czech)
  • 1. Izolace bakteriální genomové DNA; 2. Selektivní hybridizace restrikčních fragmentů (ribotypizace); 3. Makrorestrikční analýza, pulzní gelová elektroforéza; 4. PCR 16S rDNA, sekvenování genu pro druhovou identifikaci; 5. PCR-RFLP genu pro koagulázu u stafylokoků; 6. Aplikace AFLP (rep-PCR, AP-PCR) pro typizaci bakterií; 7. Indukce bakteriofága lambda a izolace fágové DNA; 8. Transdukce plazmidové DNA; 9. Konjugace u E. coli; 10. Izolace spontánních mutant rezistentních ke streptomycinu; 11. Fluktuační test; 12. Kultivace virů, růstová křivka bakteriofága; 13. Izolace plazmidu z kvasinek.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught every week.
  • Permalink: https://is.muni.cz/course/sci/spring2025/Bi8360c