Course Information

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

Mgr. Lukáš Faltinek (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Wed 16:00–18:50 C15/212, V. Sedláček
C3620/02: Mon 14:00–16:50 C15/212, M. Pelcová

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• 01. Work safety rules. Organization of laboratory exercises. Preparation of dilute solution, pipetting, and photometry.
• 02. Isolation, analysis, and determination of deoxyribonucleic acid (DNA).
• 03. Qualitative and quantitative determination of amino acids.
• 04. Quantitative determination of proteins.
• 05. Qualitative and quantitative determination of carbohydrates.
• 06. Lipids and their analysis.
• 07. Sucrase - immobilization, substrate specificity, activity determination.
• 08. pH optimum of enzyme reaction.
• 09. Enzyme kinetics.
• 10. Analytical use of enzymes.
• 11. Biological electron transfer chains.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 60% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Lukáš Faltinek (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Wed 15:00–17:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Lukáš Faltinek (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Sandra Thalerová, Ph.D. (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Thu 14:00–16:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Kristína Tošnerová (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Wed 13:00–15:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
Mgr. Šimon Vrana, Ph.D. (assistant)

Guaranteed by

doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Fri 21. 5. 8:00–12:00 C15/212, Fri 28. 5. 8:00–12:00 C15/212

• Timetable of Seminar Groups:

C3620/01: Thu 14:00–16:50 C15/212
C3620/02: Thu 17:00–19:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
prof. RNDr. Zdeněk Glatz, CSc. (seminar tutor)
Mgr. Martin Konhefr, Ph.D. (assistant)
Mgr. Martin Kryl, Ph.D. (assistant)
Mgr. Veronika Poláchová, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Wed 8:00–10:50 C15/212, P. Boublíková
C3620/02: Thu 12:00–14:50 C15/212, P. Boublíková
C3620/03: Thu 8:00–10:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Learning outcomes

Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
Mgr. Martin Konhefr, Ph.D. (assistant)
Mgr. Martin Kryl, Ph.D. (assistant)
Mgr. Veronika Poláchová, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Mon 17. 9. to Fri 14. 12. Fri 8:00–10:50 C15/212, P. Boublíková
C3620/02: Mon 17. 9. to Fri 14. 12. Thu 12:00–14:50 C15/212, P. Boublíková
C3620/03: Mon 17. 9. to Fri 14. 12. Thu 8:00–10:50 C15/212, P. Boublíková

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
Mgr. Martin Konhefr, Ph.D. (assistant)
Mgr. Martin Kryl, Ph.D. (assistant)
Mgr. Veronika Poláchová, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Mon 18. 9. to Fri 15. 12. Mon 13:00–15:50 C15/212
C3620/02: Mon 18. 9. to Fri 15. 12. Thu 8:00–10:50 C15/212, M. Konhefr
C3620/03: Mon 18. 9. to Fri 15. 12. Thu 11:00–13:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
Mgr. Martin Konhefr, Ph.D. (assistant)
Mgr. Martin Kryl, Ph.D. (assistant)
Mgr. Veronika Poláchová, Ph.D. (assistant)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Mon 19. 9. to Sun 18. 12. Mon 13:00–15:50 C15/212, M. Kryl
C3620/02: Mon 19. 9. to Sun 18. 12. Thu 8:00–10:50 C15/212, M. Konhefr
C3620/03: Mon 19. 9. to Sun 18. 12. Thu 11:00–13:50 C15/212, V. Poláchová

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Mon 13:00–15:50 C15/212
C3620/02: Thu 8:00–10:50 C15/212
C3620/03: Thu 11:00–13:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Thu 13:00–15:50 C15/212
C3620/02: Thu 10:00–12:50 C15/212
C3620/03: Wed 13:00–15:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Mon 13:00–15:50 C15/211
C3620/02: Thu 13:00–15:50 C15/211
C3620/03: Fri 10:00–12:50 C15/211

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Imobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Fri 8:00–10:50 C15/212
C3620/02: Fri 11:00–13:50 C15/212
C3620/03: Thu 13:00–15:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Mon 14:00–16:50 C15/212
C3620/02: Fri 9:00–11:50 C15/212
C3620/03: Thu 13:00–15:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Tue 12:00–14:50 C15/212
C3620/02: Tue 15:00–17:50 C15/212
C3620/03: Wed 13:00–15:50 C15/212

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Tue 7:00–9:50 C05/108
C3620/02: Thu 7:00–9:50 C05/108
C3620/03: Wed 9:00–11:50 C05/108

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Tue 16:00–18:50 C05/108
C3620/02: Wed 10:00–12:50 C05/108
C3620/03: Fri 8:00–10:50 C05/108
C3620/04: Thu 14:00–16:50 C05/108
C3620/05: Thu 11:00–13:50 C05/108
C3620/06: Wed 7:00–9:50 C05/108
C3620/07: Thu 8:00–10:50 C05/108

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Tue 8:00–10:50 C05/113
C3620/02: Tue 14:00–16:50 C05/113
C3620/03: Wed 11:00–13:50 C05/113
C3620/04: Tue 11:00–13:50 C05/113
C3620/05: Wed 8:00–10:50 C05/113

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• Návody ke cvičením - viz Studijní materiály
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)

Guaranteed by

doc. Ing. Martin Mandl, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Wed 11:00–13:50 C02/113
C3620/02: Mon 14:00–16:50 C02/113
C3620/04: Mon 17:00–19:50 C02/113
C3620/05: Tue 7:00–9:50 C02/113
C3620/06: Wed 7:00–9:50 C02/113
C3620/07: Tue 11:00–13:50 C02/113
C3620/08: Thu 12:00–14:50 C02/113

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• Návody ke cvičením - viz Studijní materiály
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)

Guaranteed by

doc. Ing. Martin Mandl, CSc.
Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Thu 11:00–13:50 C02/113, O. Janiczek
C3620/02: Tue 16:00–18:50 C02/113, P. Boublíková
C3620/03: Wed 17:00–19:50 C02/113, O. Janiczek
C3620/04: Thu 7:00–9:50 C02/113, M. Mandl
C3620/05: Wed 14:00–16:50 C02/113, M. Mandl
C3620/06: Wed 11:00–13:50 C02/113, O. Janiczek

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Heat dependence of enzyme reaction. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• Návody ke cvičením - viz Studijní materiály
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

doc. Ing. Martin Mandl, CSc.
Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: Wed 16:00–18:50 Cpl,02022, M. Mandl
C3620/02: Mon 14:00–16:50 Cpl,02022, M. Mandl
C3620/03: Wed 8:00–10:50 Cpl,02022, M. Mandl
C3620/04: Wed 13:00–15:50 Cpl,02022, O. Janiczek
C3620/05: Mon 17:00–19:50 Cpl,02022, O. Janiczek

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.

Syllabus

• Qualitative reactions of carbohydrates. Carbohydrate thin-layer chromatography.
• Isolation and identification of lipids from the egg yolk.
• Separation of lipophilic pigments using adsorption chromatography.
• Chemical reactions of amino acids and proteins. Paper chromatography of amino acids.
• Isolation of nucleoprotein from spleen.
• Gel permeation chromatography in separation of biomolecules.
• Protein determination.
• pH effect on enzyme reaction.
• Determination of the rate of enzyme reaction.
• Enzyme kinetics.
• Enzymatic determination of metabolites.
• The use of ferricyanide as an artificial electron acceptor in the electron transport chain.
• Closing test.

Literature

• MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

doc. Ing. Martin Mandl, CSc.
Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/01: No timetable has been entered into IS. M. Mandl
C3620/02: No timetable has been entered into IS. M. Mandl
C3620/03: No timetable has been entered into IS. M. Mandl
C3620/04: No timetable has been entered into IS. O. Janiczek
C3620/05: No timetable has been entered into IS. O. Janiczek
C3620/06: No timetable has been entered into IS. O. Janiczek
C3620/07: No timetable has been entered into IS. M. Mandl
C3620/08: No timetable has been entered into IS. O. Janiczek

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.

Syllabus

• Qualitative reactions of carbohydrates. Carbohydrate thin-layer chromatography.
• Isolation and identification of lipids from the egg yolk.
• Separation of lipophilic pigments using adsorption chromatography.
• Chemical reactions of amino acids and proteins. Paper chromatography of amino acids.
• Isolation of nucleoprotein from spleen.
• Gel permeation chromatography in separation of biomolecules.
• Protein determination.
• pH effect on enzyme reaction.
• Determination of the rate of enzyme reaction.
• Enzyme kinetics.
• Enzymatic determination of metabolites.
• The use of ferricyanide as an artificial electron acceptor in the electron transport chain.
• Closing test.

Literature

• MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

doc. Ing. Martin Mandl, CSc.
Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C3620/Cb30: No timetable has been entered into IS. M. Mandl
C3620/Cb34: No timetable has been entered into IS. O. Janiczek
C3620/Cb32: No timetable has been entered into IS. M. Mandl
C3620/Cb33: No timetable has been entered into IS. O. Janiczek
C3620/Cb31: No timetable has been entered into IS. M. Mandl
C3620/Cb35: No timetable has been entered into IS. O. Janiczek
C3620/Cb36: No timetable has been entered into IS. M. Mandl
C3620/Cb37: No timetable has been entered into IS. O. Janiczek

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.

Syllabus

• Qualitative reactions of carbohydrates. Carbohydrate thin-layer chromatography.
• Isolation and identification of lipids from the egg yolk.
• Separation of lipophilic pigments using adsorption chromatography.
• Chemical reactions of amino acids and proteins. Paper chromatography of amino acids.
• Isolation of nucleoprotein from spleen.
• Gel permeation chromatography in separation of biomolecules.
• Protein determination.
• pH effect on enzyme reaction.
• Determination of the rate of enzyme reaction.
• Enzyme kinetics.
• Enzymatic determination of metabolites.
• The use of ferricyanide as an artificial electron acceptor in the electron transport chain.
• Closing test.

Literature

• MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
Mgr. Michal Kuňák, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 17 fields of study the course is directly associated with, display

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 17 fields of study the course is directly associated with, display

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)

Guaranteed by

prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

At the end of the course students should be able to understand and explain - basic properties of natural substances(aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme acitivity.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms. Photosynthesis.

Literature

• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Teaching methods

laboratory courses

Assessment methods

credit course

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

C3620 Biochemistry - laboratory course

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)

Guaranteed by

doc. Ing. Martin Mandl, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites (in Czech)

NOW( C3580 Biochemistry )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Ecotoxicology (programme PřF, B-BI)

Course objectives

The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.

Syllabus

• Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. pH effect on enzyme reaction. Enzyme kinetics. Determination of enzyme activity. Inhibition of enzymes. Enzymatic determination of metabolites, substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.

Literature

• Návody ke cvičením - viz Studijní materiály
• VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
• VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info

Assessment methods (in Czech)

První cvičení je věnováno poučení o bezpečnosti práce v biochemické laboratoři. Studenti rovněž píší vstupní test, jehož úspěšné absolvování je podmínkou účasti ve cvičení. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.

Listed among pre-requisites of other courses

• C6260 Methods of Protein Separation
  !C6200 && ( C3580 && (C3600 || C3620))  

• Enrolment Statistics (recent)