C6212 Biotechnology

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (plus 2 credits for an exam). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
Ing. Matouš Čihák (lecturer)
Mgr. Vladimír Rotrekl, Ph.D. (lecturer)
Mgr. Kateřina Dadáková, Ph.D. (lecturer)
Ing. Jan Červený, Ph.D. (lecturer)
Mgr. Jiří Kučera, Ph.D. (lecturer)
Guaranteed by
doc. Ing. Martin Mandl, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: Mgr. Jiří Kučera, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 17. 2. to Sat 24. 5. Thu 11:00–12:50 C05/114
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Overview and characterization of selected modern biotechnological processes expanding existing study programs.
Learning outcomes
After completing the course, the student will be able to better understand selected modern biotechnologies utilizing microbial, plant, and animal cell cultures.
Syllabus
  • Microbial Pharmaceutical Biotechnology: Introduction to pharmaceutical production. Selection of production systems. Optimization of fermentation. Separation and purification of intracellular and extracellular products. Drying of fermentation products and final processing. Finalization of obtained products. Biotransformation. Good Manufacturing Practice documentation.
  • Animal Cell Biotechnology: Introduction to animal cells and their characteristics. Potency and differentiation, Hayflick limit, immortalized cell lines, cultivation and differentiation. Use of animal cells for pharmaceutical production. Use of stem cells in drug screening and production. Animal cells and cell therapy. Special applications and future perspectives (3D cultivation, organoids, 3D bioprinting, in vitro tissue and organ substitutes).
  • Plant and Photosynthetic Biotechnology: Introduction to plant cell physiology. Methods for the development of GMO crops, their applications, and risks. Plant-based production of biotechnologically significant compounds. Evolution of photosynthesis and endosymbiosis. Structure of pigments and energy conversion mechanisms. Carbon dioxide fixation and genetic improvement of photosynthesis. Photosynthetic microorganisms and cultivation methods. Optimization of production and control of cultivation processes.
Literature
    recommended literature
  • Applied plant cell biology : cellular tools and approaches for plant biotechnology. Edited by Peter Nick - Zdeněk Opatrný. Berlin: Springer, 2014, xiv, 481. ISBN 9783642417863. info
  • Agricultural biotechnology. Edited by Hemant Rawat. Jaipur, India: Oxford Book Co., 2008, 277 p. ISBN 9788189473235. info
  • Advances in biotechnology. Edited by S. N. Jogdand. Rev. ed. Mumbai [India]: Himalaya Pub. House, 2007, 311 p. ISBN 9788183182676. info
  • Basic biotechnology. Edited by Colin Ratledge - Bjørn Kristiansen. 3rd ed. Cambridge: Cambridge University Press, 2006, xiv, 666. ISBN 9780521549585. info
  • DORAN, Pauline M. Bioprocess engineering principles. London: Academic Press, 1995, xiv, 439 s. ISBN 0-12-220856-0. info
  • STANBURY, Peter F., Allan WHITAKER and Stephen J. HALL. Principles of fermentation technology. 2nd ed. Oxford: Pergamon, 1995, xviii, 357. ISBN 0-08-036131-5. info
  • ALEXANDER, Martin. Biodegradation and bioremediation. San Diego: Academic Press, 1994, xii, 302. ISBN 012049860X. info
Teaching methods
Lectures from selected chapters of modern biotechnology. Discussion on detailed issues.
Assessment methods
Final written exam.
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2025/C6212