C6242 Analysis of Organic Compounds II

Faculty of Science
Spring 2007
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. RNDr. Bc. Jiří Pazourek, Ph.D. (lecturer)
doc. Mgr. Jan Havliš, Dr. (lecturer)
Guaranteed by
doc. RNDr. Bc. Jiří Pazourek, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Bc. Jiří Pazourek, Ph.D.
Timetable
Wed 13:00–14:50 02004
Prerequisites
C5241 Analysis of Org.Compounds I
C5241 - Analytical chemistry of organic compounds I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The color of moleculs and its analytical exploitation. Basical indicator reactions. Immunochemistry and immunoanalytical chemistry. Analytical importance of nucleic acids (NA). Development and perrspectives of clinical chemistry.
Syllabus
  • The color of moleculs and its analytical exploitation. Basics of colorness theory (double bounds conjugation, aromatic and heteroccyclic compounds, susbstituents and molecule polarization and ionization, linear structure). Redox reaction of color molecules. Examples of applications. Basical indicator reactions (copulation, hydrogen peroxide reactions =oxidative copulation and leucobases oxidation, reactions with coenzymes NAD, tetrazolic salts and formazanes). Examples of applications. Immunochemistry and immunoanalytical chemistry. Classification of the methods (serological, precipitational, immunodiffusive with marked reactants). Competitive and noncompetitive modes, homogenic and heterogenic methods. The use of avidin and biotin. Analytical importance of nucleic acids (NA), structure and properties, target NA sequence. Polymerase chain reaction and its utilization for patogenic, forensic, prenatal diagnostics. Development and perrspectives of clinical chemistry. Consolidated laboratories and diagnostic centres. Miniaturization, chips, biosensors. Automatization in laboratory.
Literature
  • Chromý, Vratislav., Fischer, Jiří. Analytické metody v klinické chemii. 2000, Masarykova univerzita
  • STRÁNSKÝ, Zdeněk. Analýza organických sloučenin b. 1. vyd. Olomouc: Univerzita Palackého, 1981, 235 s. info
  • CHROMÝ, Vratislav. Pufrační media pro biochemické analytické systémy. 1995, 1 svazek. info
Assessment methods (in Czech)
přednáška zkouška
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006.
  • Enrolment Statistics (recent)
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