PřF:C7880 New Trends in Bioanalytical Ch - Course Information
C7880 New Trends in Bioanalytical Chemistry
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
Mgr. Marta Pelcová, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable
- Wed 16:00–17:50 B11/205
- Prerequisites
- ( C4182 Biochemistry II || C3580 Biochemistry ) && ( C6200 Biochemical Methods || C6260 Protein Separation || C4830 Inst. Methods in Biochemistry )
Basic knowledges from general biochemistry and methods of biochemical research. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Analytical Biochemistry (Eng.) (programme PřF, N-BCH)
- Analytical biochemistry (programme PřF, N-BIC)
- Bioanalytical Laboratory Diagnostics in Medicine - Bioanalyst (programme PřF, N-BIA)
- Course objectives
- The goals of the lecture are to get to students the knowledge from separation methods in biochemistry and molecular biology, which are used for macromolecules purification (proteins, nucleic acids). A content of the first part includes electromigration methods. Second part is oriented onto chromatography methods and third part is dedicated in Field Flow Fractionation, Supercritical Fluid Chromatography and Mass Spectrometry.
- Learning outcomes
- The course brings the information about the newest trends in the field of Bioanalytitcal chemistry especially in Genomics, Transcriptomics, Proteomics, Metabolomics and in other omics field. At the end of this course the students should not only theoretically understand but be able practically apply these methods in their scientific research working on bachelor, master and PhD theses and finally during their following employment as well.
- Syllabus
- A.Elektromigration Methods
- 1.Capillary Zone Electrophoresis
- 2.Izotachophoresis
- 3.Detection of Protein after Electrophoresis or Isoelectric Focusing
- 4.Blotting
- 5.Electrohpresis od Nucleic Acids
- B.Field Flow Fractionation
- C.Chromatography Methods
- 1.Microcolumn Chromatography
- 2.Gas Chromatography
- 3.Supercritical Fluid Chromatography
- D.Mass Spectrometry
- E.Application of Affinity Interaction in Protein Purification
- Literature
- SNYDER, Lloyd R., Joseph Jack KIRKLAND and Joseph L. GLAJCH. Practical HPLC method development [Snyder, 1997]. 2nd ed. New York: John Wiley & Sons, 1997, xxvi, 765. ISBN 0-471-00703-X. info
- KITSON, F. G., B. S. LARSEN and C. N. MCEWEN. Gas Chromatography and Mass Spectrometry, A Practical Guide. San Diego: Academic Press, 1996. ISBN 0-12-483385-3. info
- WEISS, Joachim. Ion chromatography. 2nd ed. Weinheim: VCH Verlagsgesellschaft, 1995, 465 s. ISBN 3-527-28698-5. info
- ROTHE, Gunter M. Electrophoresis of enzymes : laboratory methods. Berlin: Springer-Verlag, 1994, 307 s. ISBN 3540581146. info
- Handbook of capillary electrophoresis. Boca Raton: CRC Press, 1994, 649 s. ISBN 0-8493-8690-X. info
- Protein blotting : a practical approach. Edited by Bonnie S. Dunbar. Oxford: Oxford University Press, 1994, 242 s. ISBN 0199634378. info
- KUHN, Reinhard and Sabrina HOFFSTETTER-KUHN. Capillary electrophoresis : principles and practice. Berlin: Springer-Verlag, 1993, x, 375 s. ISBN 3-540-56434-9-. info
- LI, Sam F. Y. Capillary electrophoresis : principles, practice and applications. Amsterdam: Elsevier, 1993, 582 s. ISBN 0444815902. info
- FORET, František, Ludmila KŘIVÁNKOVÁ and Petr BOČEK. Capillary zone electrophoresis. Weinheim: VCH Publishers, 1993, 346 s. ISBN 3-527-30019-8. info
- LINDSAY, Sandie. High performance liquid chromatography. 2nd ed. Chichester: John Wiley & Sons, 1992, xxii, 337. ISBN 0-471-93180-2. info
- ANDREWS, Anthony T. Electrophoresis : theory, techniques and biochemical and clinical applications. 1st pub. Oxford: Clarendon Press, 1990, xv, 452 s. ISBN 0-19-854632-7. info
- Gel electrophoresis of proteins : a practical approach. Edited by B. D. Hames - D. Rickwood. 2nd ed. Oxford: Oxford University Press, 1990, xviii, 383. ISBN 0199630755. info
- High performance liquid chromatography in biotechnology. Edited by William S. Hancock. New York: John Wiley & Sons, 1990, 564 s. ISBN 0471825840. info
- MIKEŠ, O. High-performance liquid chromatography of biopolymers and biooligomers. P. B, separation of individual compound classes. Amsterdam: Elsevier, 1988, 721 s. ISBN 0444430342. info
- Teaching methods
- The two-hour lectures will be given by internal or external specialist in the respective areas.
- Assessment methods
- The final exam is in the form of written test, which contains questions from the presented lectures. The time for the test is one hour, the grades are A to F on the basis of each question classification. Examiner Prof. Janiczek.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course can also be completed outside the examination period.
The course is taught once in two years. - Teacher's information
- http://orion.chemi.muni.cz/biochem/student.htm
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2024/C7880