C8170 Enzymology - seminar

Faculty of Science
Autumn 2024
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 C05/114
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The seminar accompanies the Enzymology lecture. Home work that is allocated to the students mainly includes exercises in handling data obtained in experiments with enzymes. The solutions are discussed and explained in greater detail in the classroom sessions.
Learning outcomes
The student will become proficient in enzyme nomenclature and calculations involving units of enzyme activity and kinetic parameters of the enzymes. The student will be able to describe models for enzyme behavior and use reaction kinetics equations to model that behavior and to discriminate among alternate models. Skills in scientific calculations, processing and interpretation of experimental data will also be increased.
Syllabus
  • 1. Nomenclature of enzymes
  • 2. Stereochemistry of enzyme-catalyzed reactions
  • 3. Measurement and calculation of enzyme activity I
  • 4. Measurement and calculation of enzyme activity II
  • 5. Kinetics of one-substrate reactions, Michaelis-Menten equation I
  • 6. Kinetics of one-substrate reactions, Michaelis-Menten equation II
  • 7. Models in enzyme kinetics, parameters estimation
  • 8. Inhibition of enzyme reactions
  • 9. Multisubstrate enzyme reactions
  • 10. Determination of enzyme mechanism
  • 11. Bioanalytical aplications of enzymes I
  • 12. Bioanalytical aplications of enzymes II
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit is awarded on the basis of active participation in the seminar. Completion and submission of two excel files is required.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.

C8170 Enzymology - seminar

Faculty of Science
Autumn 2023
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 C05/114
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The seminar accompanies the Enzymology lecture. Home work that is allocated to the students mainly includes exercises in handling data obtained in experiments with enzymes. The solutions are discussed and explained in greater detail in the classroom sessions.
Learning outcomes
The student will become proficient in enzyme nomenclature and calculations involving units of enzyme activity and kinetic parameters of the enzymes. The student will be able to describe models for enzyme behavior and use reaction kinetics equations to model that behavior and to discriminate among alternate models. Skills in scientific calculations, processing and interpretation of experimental data will also be increased.
Syllabus
  • 1. Nomenclature of enzymes
  • 2. Stereochemistry of enzyme-catalyzed reactions
  • 3. Measurement and calculation of enzyme activity I
  • 4. Measurement and calculation of enzyme activity II
  • 5. Kinetics of one-substrate reactions, Michaelis-Menten equation I
  • 6. Kinetics of one-substrate reactions, Michaelis-Menten equation II
  • 7. Models in enzyme kinetics, parameters estimation
  • 8. Inhibition of enzyme reactions
  • 9. Multisubstrate enzyme reactions
  • 10. Determination of enzyme mechanism
  • 11. Bioanalytical aplications of enzymes I
  • 12. Bioanalytical aplications of enzymes II
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Autumn 2022
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 B11/335
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The seminar accompanies the Enzymology lecture. Home work that is allocated to the students mainly includes exercises in handling data obtained in experiments with enzymes. The solutions are discussed and explained in greater detail in the classroom sessions.
Learning outcomes
The student will become proficient in enzyme nomenclature and calculations involving units of enzyme activity and kinetic parameters of the enzymes. The student will be able to describe models for enzyme behavior and use reaction kinetics equations to model that behavior and to discriminate among alternate models. Skills in scientific calculations, processing and interpretation of experimental data will also be increased.
Syllabus
  • 1. Nomenclature of enzymes
  • 2. Stereochemistry of enzyme-catalyzed reactions
  • 3. Measurement and calculation of enzyme activity I
  • 4. Measurement and calculation of enzyme activity II
  • 5. Kinetics of one-substrate reactions, Michaelis-Menten equation I
  • 6. Kinetics of one-substrate reactions, Michaelis-Menten equation II
  • 7. Models in enzyme kinetics, parameters estimation
  • 8. Inhibition of enzyme reactions
  • 9. Multisubstrate enzyme reactions
  • 10. Determination of enzyme mechanism
  • 11. Bioanalytical aplications of enzymes I
  • 12. Bioanalytical aplications of enzymes II
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
autumn 2021
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 B11/335
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The seminar accompanies the Enzymology lecture. Home work that is allocated to the students mainly includes exercises in handling data obtained in experiments with enzymes. The solutions are discussed and explained in greater detail in the classroom sessions.
Learning outcomes
The student will become proficient in enzyme nomenclature and calculations involving units of enzyme activity and kinetic parameters of the enzymes. The student will be able to describe models for enzyme behavior and use reaction kinetics equations to model that behavior and to discriminate among alternate models. Skills in scientific calculations, processing and interpretation of experimental data will also be increased.
Syllabus
  • 1. Nomenclature of enzymes
  • 2. Stereochemistry of enzyme-catalyzed reactions
  • 3. Measurement and calculation of enzyme activity I
  • 4. Measurement and calculation of enzyme activity II
  • 5. Kinetics of one-substrate reactions, Michaelis-Menten equation I
  • 6. Kinetics of one-substrate reactions, Michaelis-Menten equation II
  • 7. Models in enzyme kinetics, parameters estimation
  • 8. Inhibition of enzyme reactions
  • 9. Multisubstrate enzyme reactions
  • 10. Determination of enzyme mechanism
  • 11. Bioanalytical aplications of enzymes I
  • 12. Bioanalytical aplications of enzymes II
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Autumn 2020
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 prace doma
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The seminar accompanies the Enzymology lecture. Home work that is allocated to the students mainly includes exercises in handling data obtained in experiments with enzymes. The solutions are discussed and explained in greater detail in the classroom sessions.
Learning outcomes
The student will become proficient in enzyme nomenclature and calculations involving units of enzyme activity and kinetic parameters of the enzymes. The student will be able to describe models for enzyme behavior and use reaction kinetics equations to model that behavior and to discriminate among alternate models. Skills in scientific calculations, processing and interpretation of experimental data will also be increased.
Syllabus
  • 1. Nomenclature of enzymes
  • 2. Stereochemistry of enzyme-catalyzed reactions
  • 3. Measurement and calculation of enzyme activity I
  • 4. Measurement and calculation of enzyme activity II
  • 5. Kinetics of one-substrate reactions, Michaelis-Menten equation I
  • 6. Kinetics of one-substrate reactions, Michaelis-Menten equation II
  • 7. Models in enzyme kinetics, parameters estimation
  • 8. Inhibition of enzyme reactions
  • 9. Multisubstrate enzyme reactions
  • 10. Determination of enzyme mechanism
  • 11. Bioanalytical aplications of enzymes I
  • 12. Bioanalytical aplications of enzymes II
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Autumn 2019
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 C05/114
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The seminar accompanies the Enzymology lecture. Home work that is allocated to the students mainly includes exercises in handling data obtained in experiments with enzymes. The solutions are discussed and explained in greater detail in the classroom sessions.
Learning outcomes
The student will become proficient in enzyme nomenclature and calculations involving units of enzyme activity and kinetic parameters of the enzymes. The student will be able to describe models for enzyme behavior and use reaction kinetics equations to model that behavior and to discriminate among alternate models. Skills in scientific calculations, processing and interpretation of experimental data will also be increased.
Syllabus
  • 1. Nomenclature of enzymes
  • 2. Stereochemistry of enzyme-catalyzed reactions
  • 3. Measurement and calculation of enzyme activity I
  • 4. Measurement and calculation of enzyme activity II
  • 5. Kinetics of one-substrate reactions, Michaelis-Menten equation I
  • 6. Kinetics of one-substrate reactions, Michaelis-Menten equation II
  • 7. Models in enzyme kinetics, parameters estimation
  • 8. Inhibition of enzyme reactions
  • 9. Multisubstrate enzyme reactions
  • 10. Determination of enzyme mechanism
  • 11. Bioanalytical aplications of enzymes I
  • 12. Bioanalytical aplications of enzymes II
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Autumn 2018
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 1. 10. to Fri 14. 12. Tue 10:00–11:50 B11/335
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
autumn 2017
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Mon 15:00–16:50 B11/305
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Autumn 2016
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 19. 9. to Sun 18. 12. Mon 15:00–16:50 B11/235
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Autumn 2015
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 15:00–16:50 B11/205
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Autumn 2014
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 9:00–10:50 C05/114
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
    recommended literature
  • MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
  • VODRÁŽKA, Zdeněk, Pavel RAUCH and Jan KÁŠ. Enzymologie. 2. přeprac. vyd. Praha: Vysoká škola chemicko-technologická, 1991, 245 s. ISBN 80-7080-124-7. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2014
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Wed 15:00–16:50 C05/114, P. Skládal
C8170/02: Wed 13:00–14:50 C05/114, P. Skládal
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2013
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 15:00–16:50 C05/114
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
The students will be able at the end of the course understand and create systematic names of enzymes, calculate activities of enzymes, evaluate and study enzyme kinetics, solve bioanalytical problems including calculations of assay results.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2012
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Wed 13:00–14:50 C05/114, P. Skládal
C8170/02: Wed 15:00–16:50 C05/114, P. Skládal
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
The students will be able at the end of the course understand and create systematic names of enzymes, calculate activities of enzymes, evaluate and study enzyme kinetics, solve bioanalytical problems including calculations of assay results.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2011
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Thu 10:00–11:50 C05/114, P. Skládal
C8170/02: Wed 13:00–14:50 C05/114, P. Skládal
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The students will be able at the end of the course understand and create systematic names of enzymes, calculate activities of enzymes, evaluate and study enzyme kinetics, solve bioanalytical problems including calculations of assay results.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2010
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Wed 15:00–16:50 C05/114
C8170/02: Wed 13:00–14:50 C05/114
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The students will be able at the end of the course understand and create systematic names of enzymes, calculate activities of enzymes, evaluate and study enzyme kinetics, solve bioanalytical problems including calculations of assay results.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
credit
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2009
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Fri 9:00–10:50 C05/114, P. Skládal
C8170/02: Thu 16:00–17:50 C05/114, P. Skládal
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Assessment methods
calculations and practical training related to the content of the Enzymology lecture course, credit
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2008
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Thu 9:00–10:50 C05/107
C8170/02: Thu 12:00–13:50 C05/107
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Assessment methods (in Czech)
praktické procvičování učiva probíraného na přednášce z enzymologie, zejména výpočty
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2007
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Wed 8:00–9:50 C04/211, P. Skládal
C8170/02: Mon 15:00–16:50 C04/211, P. Skládal
C8170/03: Wed 10:00–11:50 C04/211, P. Skládal
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Assessment methods (in Czech)
praktické procvičování učiva probíraného na přednášce z enzymologie, zejména výpočty
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2006
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Fri 10:00–11:50 C04/211, P. Skládal
C8170/02: Tue 13:00–14:50 C04/211, P. Skládal
C8170/03: Fri 8:00–9:50 C04/211, P. Skládal
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Assessment methods (in Czech)
praktické procvičování učiva probíraného na přednášce z enzymologie, zejména výpočty
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2005
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: Thu 10:00–11:50 Cpm,02016, P. Skládal
C8170/02: Thu 14:00–15:50 Cpm,02016, P. Skládal
C8170/03: Thu 16:00–17:50 Cpm,02016, P. Skládal
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Assessment methods (in Czech)
praktické procvičování učiva probíraného na přednášce z enzymologie, zejména výpočty
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2004
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C8170/01: No timetable has been entered into IS.
C8170/02: No timetable has been entered into IS.
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Assessment methods (in Czech)
praktické procvičování učiva probíraného na přednášce z enzymologie, zejména výpočty
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2003
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Assessment methods (in Czech)
praktické procvičování učiva probíraného na přednášce z enzymologie, zejména výpočty
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2002
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Chemistry Section – Faculty of Science
Prerequisites (in Czech)
NOW( C8160 Enzymology )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2001
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Igor Kučera, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Igor Kučera, DrSc.
Chemistry Section – Faculty of Science
Prerequisites (in Czech)
NOW( C8160 Enzymology )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2025

The course is not taught in Spring 2025

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2024

The course is not taught in Spring 2024

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2023

The course is not taught in Spring 2023

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2022

The course is not taught in Spring 2022

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2021

The course is not taught in Spring 2021

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2020

The course is not taught in Spring 2020

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2019

The course is not taught in Spring 2019

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
spring 2018

The course is not taught in spring 2018

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2017

The course is not taught in Spring 2017

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2016

The course is not taught in Spring 2016

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2015

The course is not taught in Spring 2015

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. The students will learn enzyme nomenclature. Practical calculations in enzymology - measurement of enzyme activity, calculations of enzyme kinetics. Bioanalytical applications of enzyme reactions, mechanisms of enzyme action.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
Credit; based on active participation at seminars, evaluation of examples, participation at the evaluation tests.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The students will be able at the end of the course understand and create systematic names of enzymes, calculate activities of enzymes, evaluate and study enzyme kinetics, solve bioanalytical problems including calculations of assay results.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
credit
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The students will be able at the end of the course understand and create systematic names of enzymes, calculate activities of enzymes, evaluate and study enzyme kinetics, solve bioanalytical problems including calculations of assay results.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Teaching methods
seminars, and practical training related to the content of the Enzymology lecture course, calculations
Assessment methods
credit
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://biosensor.chemi.muni.cz/edu/enzymol/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.

C8170 Enzymology - seminar

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
NOW( C8160 Enzymology )
related to the course on Enzymology
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Practical training in enzymology. Systematic nomenclature of enzymes. Measurement of enzyme activity. Calculations of enzyme kinetics. Bioanalytical applications of enzymes and related calculations.
Syllabus
  • 1. Enzyme nomenclature and enzyme activity. 2. Methods for measurement of enzyme activity I - photometric techniques. 3. Methods for measurement of enzyme activity II - oxygen sensor, pH stat, measurement of gaseous products. 4. Purification of enzymes and its evaluation. 5. Kinetics of monosubstrate reactions, Michaelis-Menten equation. 6. Determination of kinetic parameters from experimental results, integrated form of the Michaelis-Menten equation. 7. Effect of pH and temperature on enzyme kinetics. 8. Inhibition. 9. Multisubstrate reactions. 10. Cooperative effects. 11. Immobilized enzymes. 12. Bioanalytical aplications of enzymes. 13. Enzymes in immunoassay techniques.
Literature
  • KUČERA, Igor. Řešené úlohy z enzymologie (Solved problems in enzymology). Brno: Rektorát UJEP, 1987, 121 pp. info
Assessment methods (in Czech)
praktické procvičování učiva probíraného na přednášce z enzymologie, zejména výpočty
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (recent)